A microtiter plate was coated overnight with Mouse anti Bovine interferon γ antibody, clone CC330 (MCA2112) at concentration of 5μg/ml in ELISA coating buffer (BUF030A). After washing with ELISA wash buffer (BUF031A) and blocking with ELISA BSA Block (BUF032A) samples were detected using biotinylated Mouse anti Bovine interferon γ antibody, clone CC302 (MCA1783B) at 5μg/ml followed by incubation with HRP conjugated streptavidin (STAR5B) at 1/1000. Finally, TMB CORE (BUF056A) was used as substrate.Data is presented as the mean of three measurements.
STREPTAVIDIN:HRP
Detection of Rabbit anti Mouse TNFα:biotin in a sandwich ELISA with recombinant murine TNFα as the antigen and Rat anti Mouse TNFα as the coating antibody using Streptavidin:HRP (STAR5B)
Detection of Rabbit anti Mouse TNFα:biotin in a sandwich ELISA with recombinant murine TNFα (PMP24) as the antigen and Rat anti Mouse TNFα (MCA1487) as the coating antibody using Streptavidin:HRP (STAR5B)
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