Tricine buffer is categorized as a zwitterionic buffer commonly used in enzyme assays, electrophoresis, and cell culture applications. The compound tricine (N-(Tri(hydroxymethyl)methyl)glycine) is derived from tris and glycine. One of the Good buffers, tricine has a pKa value of 8.15 at 20 °C, effective for buffering at a pH range of 7.4 to 8.8. In comparison to glycine, it has a higher negative charge. This high ionic strength causes more ion movement and less protein movement. Using tricine buffer in gel electrophoresis promotes the separation of low molecular weight proteins in lower percent acrylamide gels. Visit the reagent supplier page for more information.