Bis-Tris, often referred to as bis-tris methane (C8H19NO5), is a 99% zwitterionic buffering reagent utilized in biochemical and molecular biological research. The buffer belongs to the Tris family and is suitable for a pH range of 5.8 to 7.2. It is often used in gel preparation and as a running buffer for various types of electrophoresis. It is also used as a buffer in cell culture, biochemical purification, hemoglobin fractionation, analytical chromatography, and as a diagnostic reagent. It should be noted that bis-tris may form complexes with several common metals, including Pb (II) and Cu (II). Visit the supplier page for more reagent information, including details on formulation and format.