Anti-SAG antibodies are used in the immunodetection of the protein S-antigen visual arrestin. In humans, the canonical protein has a reported length of 405 amino acid residues and a mass of 45.1 kDa. Its subcellular localization is in the cell projections and membrane. It is notably expressed in retina, in the proximal portion of the outer segment of rod photoreceptor cells. A member of the Arrestin protein family, SAG is known to bind to photoactivated, phosphorylated RHO and terminates RHO signaling via G-proteins by competing with G-proteins for the same binding site on RHO (By similarity).
The SAG marker can be used to identify Eye Photoreceptor Cells.* Synonyms for this target antigen include RP96, S-arrestin, 48 kDa protein, S-antigen; retina and pineal gland (arrestin), arrestin 1, and RP47. SAG gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species. A number of SAG antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. Immunocytochemistry, Immunofluorescence, and Immunohistochemistry are also common applications.
*HuBMAP Human Reference Atlas v1.4