Creative BioLabs has extensive experience in antibody humanization. Humanization is important for reducing the immunogenicity of monoclonal antibodies derived from xenogeneic sources (commonly rodent) and for improving their activation of the human immune system. Since the development of the hybridoma technology, a large number of rodent monoclonal antibodies with specificity for antigens of therapeutic interest have been generated and characterized. Rodent antibodies are highly immunogenic in humans, which limits their clinical applications, especially when repeated administration is required. Importantly, they are rapidly removed from circulation and can cause systemic inflammatory effects as well. As a means of circumventing these problems, we have developed three antibody humanization strategies that can preserve the specificity and affinity of the antibody toward the antigen whereas significantly or completely eliminate the immunogenicity of the antibody in humans. The first approach is CDR grafting and the second approach is chain shuffling. These two methods are all based on phage display of humanized scFv variants and selection of high-affinity humanized binders through bio-panning. The third method, humanized IgG library screening, is somehow unique. We will make a library of humanized whole IgG to be displayed on the surface of mammalian cells and then high-affinity binders will be sorted by FACS.
As a leading supplier for services in the biotechnology field, Creative BioMart Services for membrane protein extraction, isolation and screening are open to any project, with a focus on the demanding class of membrane proteins. Our service includes a thorough discussion and counseling of your project, so that the methods used provide optimal conditions for your protein of interest.
We use a kind of particle to provide an alternative to living cells, membrane preparations, and detergent-solubilized proteins by offering concentrated membrane proteins in their native conformation. Particles can be used in standard membrane protein assays and are compatible with a wide variety of detection platforms.
Creative BioMart provides technical support including experimental protocols and strict quality control using up to 16 metrics per batch. Any service package can be booked on its own, or in conjunction with others. Please contact us for more information
Our staff scientists have extensive experience in screening of phage display peptide, cDNA and scFv/Fab libraries. In particular, by conducting library screening, panning, for 4 cycles, we normally get scFv/Fab antibodies of an affinity of 10-7 By constructing serial sub-libraries of the isolated scFv/Fab antibodies, Creative Biolabs' proprietary protocol allows increase of the affinity of the scFV antibodies from 10-8 to 10-9. We have successfully obtained a scFV antibody that has an extremely high affinity of 10-12, whose binding to the antigen is essentially irreversible.