Screening / Development Services

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Service: ELISA and Cell-Based Assay Development (Cell Cycle, Viability)

Service: ELISA and Cell-Based Assay Development (Cell Cycle, Viability)

Altogen Labs

  • Catalog Number: CRO-10012
  • Description: ELISA and Cell-Based Assay Development (Cell Cycle, Viability) ELISA and Cell-Based Assays Altogen Labs can develop a cell-based assay to quantify the effect of a client’s proprietary compound by quantitation of specific biomarkers, cytokines, or phosphoproteins in cell culture supernatant or whole-cell lysates. Altogen Labs uses a cell-based assay to analyze customer samples with a robust ELISA-based assay. The activity of the compound of interest is tested on a panel of 16 intracellular Ser/Thr kinases and 22 Tyr kinases, using phospho-specific antibodies by the determination of Substrate-specific phosphorylation activity induced by kinase activation, IC50 concentrations of Ser/Thr kinases and Tyr kinases.
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PhaseZERO® Enzyme activity assay

PhaseZERO® Enzyme activity assay

Asterand plc

  • Catalog Number:
  • Description: Determine acetylcholinesterase activity in frozen cerebral cortex. Profiling compounds at native human receptors to support pre-clinical go/no go decisions.
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GPCR Screening Service

GPCR Screening Service

ChanTest

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  • Catalog Number: Service
  • Description: Functional, validated cell-based assays for Drug Discovery
    ChanTest’s GPCR screening panel includes over 125 targets, and includes receptors from all major GPCR classes. All assays are fully validated and have been designed to provide high quality screening and profiling data for both drug discovery and safety assessments.

    Instrumentation
    FLIPRTETRA®, FlexStation® 3

    Readouts
    cAMP, calcium, membrane potential, binding

    GPCR Screening
    Cell-based screens performed with calcium-sensitive or membrane-potential-sensitive dyes. Gs- and Gi-coupled GPCRs are assayed via cAMP determinations using either a cell-based real time cAMP assay or other validated cAMP assay platform. EC50 / IC50 determinations on confirmed actives are offered as a follow-up option. All screens include positive controls and a comprehensive report.

    GPCR Profiling
    Compound profiling against multiple receptors to determine specificity or off-target activity.

    Mode of Action Studies
    Full characterization of orthostatic and allosteric ligands.

    GPCR Functional Selectivity
    Measurement of GPCR functional selectivity and differentiation from assay format artefacts.

    ChanTest Compound Library
    Our diverse, custom-made compound library consists of 12,600 compounds (40 384-well plates). Library screen turnaround is one business week and includes EC50 / IC50 follow-up of confirmed actives identified in the screen.

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SUBCLONING, SCREENING, EXPANSION and CRYOPRESERVATION of customer provided cell line

SUBCLONING, SCREENING, EXPANSION and CRYOPRESERVATION of customer provided cell line

Rockland Immunochemicals, Inc.

  • Catalog Number: MAB-006
  • Description: Clones that require subcloning and further processing are screened against a cell line provided by the client.
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shRNA screening/validation service

shRNA screening/validation service

Vector BioLabs Inc

  • Catalog Number: 90500
  • Description: Custom screening of working shRNA sequences from 5-10 shRNA candidates in a cell based assay.
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  • Altogen Labs
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  • ChanTest
  • Rockland Immunochemicals, Inc.
  • Vector BioLabs Inc