BfaI from New England Biolabs

Product Specs
ItemBfaI
CompanyNew England Biolabs
Price $80.00 Inquire
Catalog NumberR0568S
Quantity500 units
Concentration5 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/TAG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Her9 is required for the migration, differentiation, and survival of neural crest cellsDifferentiationJanuary 16, 2026Cagney E. Coomer, Sumanth Manohar, Evelyn M. Turnbaugh, Ann C. Morrisand mutant alleles were identified by RFLP of PCR products using the BfaI restriction enzyme (NEB: R0568S) as described (Coomer et al., 2020). The restriction digests were visualized on a 2% agarose gel. RT-PCR

(2) Her9 is required for the migration, differentiation, and survival of neural crest cellsbioRxivJuly 25, 2025Cagney E. Coomer, Sumanth Manohar, Evelyn M. Turnbaugh, Ann C. Morrisand mutant alleles were identified by RFLP of PCR products using the BfaI restriction enzyme (NEB: R0568S) as described (Coomer et al., 2020). The restriction digests were visualized on a 2% agarose gel. RT-PCR

(3) A stepwise mode of TGFβ-SMAD signaling and DNA methylation regulates naïve-to-primed pluripotency and differentiationNature CommunicationsNovember 22, 2024Bingnan Zhao, Xiuwei Yu, Jintong Shi, Shuangyu Ma, Shizhao Li, Haitao Shi, Shoubing Xia, Youqiong Ye, Yongchun Zhang, Yanhua Du, et al.(TIANGEN) (Cat # DP304). 4 μg gDNA was cut with a methylation-insensitive restriction enzyme BfaI (NEB, R0568) at 37 °C overnight. 500 ng of purified BfaI cut products was heat denatured at 99 °C for 10 min and

(4) Regulation of T helper cell differentiation by the interplay between histone modification and chromatin interactionImmunityMay 14, 2024Shuai Liu, Yaqiang Cao, Kairong Cui, Gang Ren, Tingting Zhao, Xuezheng Wang, Danping Wei, Zuojia Chen, Rama Krishna Gurram, Chengyu Liu, et al.,New England Biolabs,Cat# R0639L CviAII,New England Biolabs,Cat# R0640L BfaI,New England Biolabs,Cat# R0568L MluCI,New England Biolabs,Cat# R0538L NlaIII,New England Biolabs,Cat# R0125L Klenow fragment (3’->5’

(5) Antagonistic action of GPS2 and KDM1A at enhancers governs alternative macrophage activation by interleukin 4Nucleic Acids ResearchFebruary 22, 2023Zhiqiang Huang, Astradeni Efthymiadou, Ning Liang, Rongrong Fan, Eckardt TreuterDNA was purified by phenol-chloroform buffer and further digested with the second enzyme BfaI (NEB, R0568L) at 37°C overnight. The second restriction enzyme was inactivated by incubating the mix for 20 min at

(6) GSK3 inhibition rescues growth and telomere dysfunction in dyskeratosis congenita iPSC-derived type II alveolar epithelial cellseLifeMay 13, 2022Fernandez RJ, Gardner ZJG, Slovik KJ, Liberti DC, Estep KN, Yang W, Chen Q, Santini GT, Perez JV, Root S, et al.FSP995H-INCL, "Peptide, recombinant protein",CviAII,NEB,CAT# R0640L, "Peptide, recombinant protein",BfaI,NEB,CAT# R0568L, "Peptide, recombinant protein",MseI,NEB,CAT# R0525L, "Peptide, recombinant protein",NdeI,NEB,CAT#

(7) Quantitative spatial and temporal assessment of regulatory element activity in zebrafisheLifeNovember 19, 2021Shipra Bhatia, Dirk Jan Kleinjan, Kirsty Uttley, Anita Mann, Nefeli Dellepiane, Wendy A Bickmore#68310, Recombinant DNA reagent,NlaIII(enzyme),NEB,R0125S, Recombinant DNA reagent,BfaI(enzyme),NEB,R0568S, Recombinant DNA reagent,DpnII(enzyme),NEB,R0543S, "Chemical compound, drug",PTU (1-phenyl2-thio-urea),Sigma-Aldrich,S515388, "Chemical

(8) Primary Cilia Formation Does Not Rely on WNT/β-Catenin SignalingFrontiers in cell and developmental biologyJanuary 1, 2021Bernatik O, Paclikova P, Kotrbova A, Bryja V, Cajanek L.forward: cagtgccaggggaagaag, and AXIN2 reversed: gtcttggtggcaggcttc. PCR products were cut by BfaI (R0568S, NEB) in case of LRP5 KO and Hpy188III (R0622S, NEB) for AXIN2 KO screening, respectively. Successful disruption

(9) Her9/Hes4 is required for retinal photoreceptor development, maintenance, and survivalScientific ReportsDecember 1, 2020Cagney E. Coomer, Stephen G. Wilson, Kayla F. Titialii-Torres, Jessica D. Bills, Laura A. Krueger, Rebecca A. Petersen, Evelyn M. Turnbaugh, Eden L. Janesch, Ann C. Morriscarrying a 1 bp insertion was identified by RFLP due to the introduction of a BfaI restriction site (NEB: R0568S). Genomic DNA from whole embryos or from tail clips was extracted and amplified by PCR as described

(10) Yellow sea mediated segregation between North East Asian Dryophytes speciesPLOS ONEJune 24, 2020Amaël Borzée et al.Quick-DNA Miniprep™ Plus Kit (Zymo #D4069), then 500 ng of genomic DNA was digested by 0.5 U of BfaI (NEB, Cat# R0568S) and MboI (NEB, Cat# R0147S) with 10X CutSmart buffer (NEB, Cat# B7204S) at 37˚C for 1 hour. We chose

(11) Tet inactivation disrupts YY1 binding and long-range chromatin interactions during embryonic heart developmentNature communicationsSeptember 20, 2019Fang S, Li J, Xiao Y, Lee M, Guo L, Han W, Li T, Hill MC, Hong T, Mo W, et al.the nuclei. For the Mpdu1 loci, DpnII (NEB R0543) was used for the primary digestion and BfaI (NEB R0568S) was used for the secondary digestion. PCR was performed using the Roche Expand Long Template polymerase

(12) YY1 Is a Structural Regulator of Enhancer-Promoter LoopsCellDecember 14, 2017Weintraub AS, Li CH, Zamudio AV, Sigova AA, Hannett NM, Day DS, Abraham BJ, Cohen MA, Nabet B, Buckley DL, et al.the second digestion was performed overnight in 450 μL with 50 units of restriction enzyme. BfaI (NEB R0568S) was used for Etv4 and CviQI (NEB R0639S) was used for Raf1. Samples were phenol-chloroform extracted

(13) Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposonsNature ProtocolsApril 1, 2014Zoltán Ivics et al.(New England Biolabs, cat no. R0179S) • BfaI restriction endonuclease (New England Biolabs, cat no. R0568S) • DpnII restriction endonuclease (New England Biolabs, cat no. R0543S) • Taq DNA polymerase, provided

(14) Germline transgenesis in rodents by pronuclear microinjection of Sleeping Beauty transposonsNature ProtocolsApril 1, 2014Zoltán Ivics et al.(New England Biolabs, cat no. R0179S) • BfaI restriction endonuclease (New England Biolabs, cat no. R0568S) • DpnII restriction endonuclease (New England Biolabs, cat no. R0543S) • Taq DNA polymerase, provided

(15) Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposonsNature ProtocolsApril 1, 2014Zoltán Ivics et al.contact with skin, eyes and airways. • BfaI restriction endonuclease (New England Biolabs, cat. no. R0568S) • DpnII restriction endonuclease (New England Biolabs, cat no. R0543S) • Taq DNA polymerase, provided

(16) Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCRJournal of Visualized ExperimentsFebruary 1, 2013Callie L. Janik, Timothy K. StarrGenomic DNA from Tumors Name of the reagent Company Catalogue number BfaI New England Biolabs R0568S NlaIII New England Biolabs R0125S MinElute 96 well plates Qiagen 28051 QIAvac 96 Vacuum manifold Qiagen 19504 Multi-MicroPlate

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BfaI

BfaI from New England Biolabs

Sequence

New England Biolabs

Citations (16)

Reviews

BfaI from New England Biolabs

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