ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate SEL002 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate
CompanyR&D Systems, a Bio-Techne Brand
Price $200.00Supplier Page View Company Product Page
Catalog NumberSEL002
Quantity1 Pack (for 5 Plates)
ApplicationsELISpot
Assay Time3 hours 35 mins to 4 hours 50 mins**
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date

Specifications/Features

Available conjugates: Sizes Available: 1 Pack (for 5 Plates)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) ULK1 drives NDP52-mediated selective autophagic degradation of MHC-I to promote immune evasion in HPV-positive head and neck cancerbioRxivMarch 20, 2026Lexi Vu, Nicholas S. Giacobbi, Mohamed I. Khalil, Canchai Yang, William J. Eckerman, Evelyn Gomez Recinos, Johnathon D. Garber, Hyomin Son, Prerna Chahal, Dara M. Villa, et al.SEL485), visualized using the ELISpot Blue Color Module (Strep-AP and BCIP-BNBT) (R&D Systems, No. SEL002), and imaged using the ImmunoSpot Analyzer (CTL). CTL files were analyzed by NIH ImageJ. A consistent

(2) NR2F6 deletion revives CAR-T cell function and induces antigen-agnostic immune memory in solid tumorsNature CommunicationsFebruary 27, 2026Dominik Humer, Victoria Klepsch, Dietmar Rieder, Isabel Hölzl, Daniel Schreiber, Viktor Lang, Jiří Koutník, Sebastian Peer, Tajana Sajinovic, Viana Wille, et al.Following another set of washes, the Granzyme B plates were incubated with BCIP/NBT substrate (BioTechne, SEL002) for 1 h and then washed thoroughly with tap water. All plates were left to dry before further analysis.

(3) Rabies vaccination induces a CD4+ TEM and CD4+CD8+ TEMRA TH1 phenotype in dogsPloS oneJanuary 1, 2025Lang HP, Almeer FF, Jenkins MK, Friedenberg SG.and incubated at 4°C overnight. Plates were washed and developed using the ELISpot Blue Color Module (SEL002, R&D Systems). Spots were counted using a CTL ImmunoSpot® Analyzer, and IFNγ spot numbers between RABV-G-

(4) Multiple sclerosis patient-derived spontaneous B cells have distinct EBV and host gene expression profiles in active diseaseNature microbiologyJune 1, 2024Soldan SS, Su C, Monaco MC, Yoon L, Kannan T, Zankharia U, Patel RJ, Dheekollu J, Vladimirova O, Dowling JW, et al.proceeded to color development with ELISpot Blue Color Module following the manufacturer’s protocol (SEL002, R&D Systems, Inc., Minneapolis, MN). Color development was stopped with a water wash and the plate

(5) A prospective CSFV-PCV2 bivalent vaccine effectively protects against classical swine fever virus and porcine circovirus type 2 dual challenge and prevents horizontal transmissionVeterinary researchJuly 11, 2023Chen JY, Wu CM, Chia MY, Huang C, Chien MS.of detection antibody to each well and incubate overnight at 4 °C. The ELISpot blue colour module (SEL002, R&D systems) was used for spot colour development. A positive reaction is indicated by the blue spots,

(6) Evaluation of the Interactions between Mumps Virus and Guinea PigJournal of VirologyApril 27, 2023Maja Lang Balija, Adela Štimac, Tanja Košutić Gulija, Andrea Gudan Kurilj, Ana Bekavac, Ante Plećaš, Beata Halassy, Maja Jagušić, Dubravko Forčićto each well. Following 2 h of incubation and washing, alkaline phosphatase-conjugated streptavidin (SEL002; R&D Systems Inc., Minneapolis, MN) was diluted 1:100, and then 100 μL was added to each well for 1

(7) Unstable EBV latency drives inflammation in multiple sclerosis patient derived spontaneous B cellsResearch squareFebruary 1, 2023Soldan S, Su C, Monaco MC, Brown N, Clauze A, Andrada F, Feder A, Planet P, Kossenkov A, Schäffer D, et al.proceeded to color development with ELISpot Blue Color Module following the manufacturer’s protocol (SEL002, R&D Systems, Inc., Minneapolis, MN). Color development was stopped with a water wash and the plate

(8) Adjuvant effect of dendritic cells activator Imiquimod in genetic immunization with HIV-1 p55 GagJournal of immunological techniques in infectious diseasesJanuary 1, 2023Alves JM, Inyushin M, Tsytsarev V, Roldan-Kalil JA, Miranda-Valentin E, Maldonado-Martinez G, Ramos-Feliciano KM, Hunter-Mellado R.

(9) Evaluation of the Delivery of a Live Attenuated Porcine Reproductive and Respiratory Syndrome Virus as a Unit Solid Dose Injectable VaccineVaccinesOctober 30, 2022Hayhurst E, Rose E, Pedrera M, Edwards JC, Kotynska N, Grainger D, Sadigh Y, Flannery J, Bonnet L, Ritwik R, et al.

(10) Early human B cell signatures of the primary antibody response to mRNA vaccinationProceedings of the National Academy of Sciences of the United States of AmericaJuly 12, 2022Kardava L, Rachmaninoff N, Lau WW, Buckner CM, Trihemasava K, Blazkova J, Lopes de Assis F, Wang W, Zhang X, Wang Y, et al.

(11) Memory B Cells Induced by Sputnik V Vaccination Produce SARS-CoV-2 Neutralizing Antibodies Upon Ex Vivo RestimulationFrontiers in ImmunologyFebruary 24, 2022Maria G. Byazrova, Sergey V. Kulemzin, Ekaterina A. Astakhova, Tatyana N. Belovezhets, Grigory A. Efimov, Anton N. Chikaev, Ilya O. Kolotygin, Andrey A. Gorchakov, Alexander V. Taranin, Alexander V. Filatovwashes with 0.05% Tween-20/PBS, streptavidin alkaline phosphatase conjugate (R&D Systems, Cat. No. SEL002) was added at a 1:60 dilution and the plates were further incubated for 2 hours at room temperature.

(12) Messenger RNA expressing PfCSP induces functional, protective immune responses against malaria in micenpj VaccinesDecember 1, 2021Katherine L. Mallory et al.developed with the ELISpot Blue Color Module according to the manufacturer’s instructions (R&D Systems SEL002) and allowed to dry completely before analysis. Spot counting was performed using an AID ELISpot Reader

(13) Pre-vaccination and early B cell signatures predict antibody response to SARS-CoV-2 mRNA vaccinemedRxiv : the preprint server for health sciencesJuly 7, 2021Kardava L, Rachmaninoff N, Lau WW, Buckner CM, Trihemasava K, de Assis FL, Wang W, Zhang X, Wang Y, Chiang CI, et al.

(14) Modifying bacterial flagellin to evade Nod-like Receptor CARD 4 recognition enhances protective immunity against SalmonellaNature microbiologyDecember 1, 2020Tourlomousis P, Wright JA, Bittante AS, Hopkins LJ, Webster SJ, Bryant OJ, Mastroeni P, Maskell DJ, Bryant CE.

(15) Synthetic DNA Delivery of an Engineered Arginase Enzyme Can Modulate Specific Immunity In VivoMolecular therapy. Methods & clinical developmentSeptember 11, 2020Khoshnejad M, Perales-Puchalt A, Dia Y, Xiao P, Patel A, Xu Z, Zhu X, Yun K, Baboo I, Qureshi R, et al.

(16) Severe immunosuppression and not a cytokine storm characterizes COVID-19 infectionsJCI InsightSeptember 3, 2020Kenneth E. Remy, Monty Mazer, David A. Striker, Ali H. Ellebedy, Andrew H. Walton, Jacqueline Unsinger, Teresa M. Blood, Philip A. Mudd, Daehan J. Yi, Daniel A. Mannion, et al.(M8IPS4510). Spots were detected using a colorimetric reagent kit (Strep-AP and BCIP-NBT, R&D Systems, SEL002). Following development, images were captured and analyzed on CTL ImmunoSpot 7.0 plate reader and software. The

(17) T Cell- and Monocyte-Specific RNA-Sequencing Analysis in Septic and Nonseptic Critically Ill Patients and in Patients with CancerJournal of immunology (Baltimore, Md. : 1950)October 1, 2019Washburn ML, Wang Z, Walton AH, Goedegebuure SP, Figueroa DJ, Van Horn S, Grossman J, Remlinger K, Madsen H, Brown J, et al.a colorimetric reagent kit (Strep-AP and BCIP-NBT, R&D Systems, Minneapolic MO, USA, catalog number SEL002). ELISpot images were captured and analyzed on Cellular Technologies Ltd (Cleveland, OH, USA) ImmunoSpot

(18) Therapeutic Mucosal Vaccination of Herpes Simplex Virus 2-Infected Guinea Pigs with Ribonucleotide Reductase 2 (RR2) Protein Boosts Antiviral Neutralizing Antibodies and Local Tissue-Resident CD4 ⁺ and CD8 ⁺ T _RM Cells Associated with Protection against Recurrent Genital HerpesJournal of VirologyMay 1, 2019Ruchi Srivastava et al.to each well. Following a 2-h incubation and washing, alkaline phosphatase-conjugated streptavidin (SEL002, R&D Systems Inc., Minneapolis, MN) was diluted 1:100 and wells were incubated with 100 ␮l for 1 h at

(19) Protective immunity by an engineered DNA vaccine for Mayaro virusPLoS neglected tropical diseasesFebruary 1, 2019Choi H, Kudchodkar SB, Reuschel EL, Asija K, Borole P, Ho M, Wojtak K, Reed C, Ramos S, Bopp NE, et al.

(20) Enhancement of Immune Responses by Guanosine-Based Particles in DNA Plasmid Formulations against Infectious DiseasesJournal of immunology researchJanuary 1, 2019Santos S, Ramírez M, Miranda E, Reyes N, Martínez O, Acosta-Santiago M, Rivera JM, Otero M.

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ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate from R&D Systems, a Bio-Techne Brand

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