TRANCE/TNFSF11/RANK L Protein 462-TEC-010 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemTRANCE/TNFSF11/RANK L Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $454.00Supplier Page View Company Product Page
Catalog Number462-TEC-010
SpeciesMouse
Molecule NameTRANCE/TNFSF11/RANK L
Size10 ug
FormatLyophilized from a 0.2 µm filtered solution in NaH2PO4, NaCl and EDTA with BSA as a carrier protein
Purity>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
ApplicationsBioactivity
Conjugate/TagUnconjugated
Source/Expression SystemE. coli-derived Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Sizes Available: 10 ug

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Selective disruption of microtubule formation at the nuclear envelope impairs the bone resorption capacity of osteoclastsJournal of Cell ScienceJune 15, 2026Silvia Vergarajauregui, Samantha Panea, Jakob O. Oltmanns, Ulrike Steffen, Felix B. Engel(Thermo Fisher Scientific, 41061029) supplemented with 10% heat-inactivated FBS and 50 ng/ml RANK-L (R&D Systems, 462-TEC-010). Medium was replaced every 2 days with large multinucleated osteoclasts formed at day 4. siRNA transfection RAW264.7

(2) Optimizing in vitro osteoclastogenesis: bone marrow-derived macrophages differentiation and cell density as critical determinantsPeerJMarch 25, 2026Jie Li, Xinyi Sun, Changqing Yan, Weiwei Zhao, Dandan Liu, Yang Liu, Shuguo Zhengcontaining 30 ng/mL recombinant mouse M-CSF (576406; BioLegend) and 50 ng/mL recombinant mouse RANKL (462-TEC-010; R&D Systems). The non-adherent cells were seeded in 24-well or 12-well plates at densities of 0.28

(3) Exosomal miR-484 from lung adenocarcinoma promotes osteoclastogenesis and osteolytic bone metastasis by targeting PECAM1Discover OncologyFebruary 6, 2026Zhongkai Tong, Xiaoxiao Zhu, Mengqing Hu, Cenli Wang, Xiaofei Liang, Chunli Wu, Zhenyan Li, Lin He, Jian Li, Zhenyue Ye, et al.cultured under the same conditions and differentiated into OCs using RANKL (50 ng/mL; R&D Systems, #462-TEC-010) and Macrophage colony-stimulating factor (M-CSF; 25 ng/mL; R&D Systems, #416-ML-050) as positive controls

(4) Cuproptosis promotes inflammatory osteolysis via GYS1-mediated glycogen metabolismInt J Oral SciFebruary 3, 2026Lu Zhou, Hanqing Mao, Yuanhao Wen, Zhi Chen, Lu ZhangPeproTech, 315-02) and 50 ng/mL of receptor activator of nuclear factor κB ligand (RANKL, R&D Systems, 462-TEC-010). The cells were cultured at 37 °C for 7-10 days until maturation. The assay was concluded on the same

(5) Vildagliptin and Omarigliptin Differentially Bind to DPP‐4 Homodimers and Modulate Osteoclast‐Mediated Bone ResorptionComprehensive PhysiologyFebruary 1, 2026Ratchaneevan Aeimlapa, Jiraporn Panmanee, Jarinthorn Teerapornpuntakit, Kannikar Wongdee, Jirawan Thongbunchoo, Nattapon Panupinthu, Saovaros Svasti, Nattayaporn Apaijai, Piangkwan Sa‐nguanmoo, Siriporn Chattipakorn, et al.with 10 ng/mL M‐CSF and 10 ng/mL receptor activator of nuclear factor‐κB ligand (RANKL; catalog no. 462‐TEC‐010; R&D Systems) to induce osteoclast differentiation. Multinucleated osteoclasts were observed on days

(6) Paroxetine as a Therapeutic Agent in Inflammatory Osteolysis: Mechanistic Insights and EfficacyDrug Design, Development and TherapyJanuary 27, 2026Junming Huang, Zhipeng Wang, Jun Liu, Jihong Zhang, Kui Deng, Shanhu Huang, Song Zhoufor inflammatory osteolysis management. Materials and Methods Reagents Recombinant mouse RANKL (462-TEC-010) and M-CSF (416-ML-010) were obtained from Bio-Techne (Minneapolis, MN). Paroxetine (HY-122272), SC79

(7) Paroxetine as a Therapeutic Agent in Inflammatory Osteolysis: Mechanistic Insights and EfficacyDrug Des Devel TherJanuary 27, 2026Junming Huang, Zhipeng Wang, Jun Liu, Jihong Zhang, Kui Deng, Shanhu Huang, Song Zhoufor inflammatory osteolysis management. Materials and Methods Reagents Recombinant mouse RANKL (462-TEC-010) and M-CSF (416-ML-010) were obtained from Bio-Techne (Minneapolis, MN). Paroxetine (HY-122272), SC79

(8) Disrupted bone microenvironment and immune recovery following total body irradiation in a murine modelCell Death & DiseaseDecember 13, 2025Tibor Sághy, Priti Gupta, Karin Horkeby, Petra Henning, Claes Ohlsson, Carmen Corciulo, Marie K. Lagerquist, Andrei S. Chagin, Cecilia Engdahlmedia. All cultivations were stimulated with 30 ng/mL M-CSF for survival, 4 ng/mL RANKL (R&D Systems; 462-TEC-010) to induce osteoclast differentiation, and additionally stimulated with 0.5 ng/ml TGF-β1 (R&D Systems;

(9) Nature-inspired IL-1 targeted therapy to treat chronic inflammatory diseasesMolecular TherapyDecember 3, 2025Yeon-Suk Yang, Mi-Jeong Kim, Sachin Chaugule, Emma Mayer, Ngoc DeSouza, Hong Ma, Jun Xie, Ki-Young Lee, Shaoguang Li, Ellen Gravallese, et al.osteoclasts 1 day later in the presence of M-CSF (20 ng/mL) and RANKL (10 ng/mL; R&D Systems, catalog no. 462-TEC-010). Cells were stimulated with inflammatory cytokines for 1 day. GFP expression was assessed by immunoblotting

(10) Isonardosinone suppresses osteoclastogenesis through TRAF2/TRAF6-mediated disruption of calcium signaling and coordinate inhibition of MAPK/NF-κB pathwaysBBA AdvancesDecember 1, 2025Jielong Huang, Yang LV, Chujie Wang, Jinfang Wang, Wei Deng, Jiyao Luan, Zitong Tangfrom Thermo Fisher Scientific (China) Limited (Shanghai, China). Recombinant mouse TRANCE/RANKL (Cat #462-TEC-010) and recombinant mouse M-CSF protein (Cat #416-ML-050) were sourced from R&D Systems (Shanghai, China).

(11) A novel HDAC1-specific inhibitor prevents estrogen deficiency-induced osteoporosis in mice by inhibiting osteoclast functionArchives of gerontology and geriatricsDecember 1, 2025Yuan H, Bao Y, Li L, Luan Y, Li F.

(12) BPAG1 promotes osteoclastogenesis and postmenopausal osteoporosis via activating ERK signaling pathwayiScienceNovember 21, 2025Yufeng Zhang, Han Zheng, Li Yang, Yi Wang, Liang Zhangsupplemented with 30 ng/mL M-CSF (R&D Systems, Cat# 416-ML-010) and 50 ng/mL RANKL (R&D Systems, Cat#462-TEC-010) to induce differentiation. The culture medium was refreshed every other day. The drug experiment used

(13) Preclinical Study of Pain Neuropeptide Expression in Murine Sensory Neurons Induced by Irradiated Osteoclasts in the Context of Stereotactic Body Radiation TherapyCellsAugust 27, 2025Sun H. Park, Megan Peters, Caleb Aguayo, Michael K. Farris, Ryan T. Hughes, Joseph Moore, Michael T. Munley, Kaitlyn E. Reno, Jeffrey A. Foster, Jean Gardin, et al.containing 35 ng/mL of murine recombinant receptor activator of nuclear factor kappa-beta ligand (RANKL) (462-TEC-010, R&D systems Inc., Minneapolis, MN, USA). Differentiated osteoclasts (identified by bright field microscopy

(14) Skimmianine Attenuates Osteoclast Activity by Suppressing ERp57-Driven Calcium Oscillations/Calcineurin/Nfatc1 Signalling in Postmenopausal OsteoporosisJ Cell Mol MedAugust 12, 2025Yongshuang Lv, Xin Zhang, Qizhen Lu, Yi Zhou, Weiyi Wang, Maosheng Yang, Tao Yuan, Yikai Liu, Shui Sun, Ziqing Lifactor (M‐CSF, 576406; BioLegend) and receptor activator of nuclear factor‐κB (RANK) ligand (RANKL, 462‐TEC‐010, R&D Systems). TRAP staining reagents include sodium tartrate dibasic dihydrate (6106‐24‐7, Sigma‐Aldrich),

(15) Visomitin Attenuates Pathological Bone Loss by Reprogramming Osteoclast Metabolism via the STAT3/LDHB AxisResearch (Wash D C)July 22, 2025Peihua Yuan, Zhenhua Yuan, Zhenhua Feng, Haotian Yang, Hongwei Xue, Hongwei Xie, Haoming Wang, Haoming Wang, Ying Liu, Hongpu Song, et al.factor (M-CSF) (Cat. No. CB34) was supplied by Novoprotein; Recombinant soluble mouse RANKL (Cat. No. 462-TEC-010) were purchased from R&D Systems; Total Antioxidant Capacity (T-AOC) Assay Kit (Cat. No. S0121), ROS

(16) Receptor activator of nuclear factor-kappa B ligand-derived microglia healing peptide 1-AcN inhibits osteoarthritis progression in miceArthritis research & therapyJuly 9, 2025Fukuda Y, Shimamura M, Etani Y, Noguchi T, Kurihara T, Goshima A, Miura T, Hirao M, Ochiai N, Ju N, et al.

(17) Urolithin A Attenuates Periodontitis in Mice via Dual Anti-Inflammatory and Osteoclastogenesis Inhibition: A Natural Metabolite-Based Therapeutic StrategyMoleculesJuly 7, 2025Yishu Xia, Danni Wu, Linyi Zhou, Xinyu Wu, Jianzhi Chen(Cat# ZLI-9018) was obtained from ZSGB-BIO (Beijing, China). RANKL osteoclast induction medium (Cat# 462TEC010) was obtained from R&D Systems (Minneapolis, MN, USA). 4.2. Cell Culture After thawing, mouse fibroblasts

(18) Mechanical control of osteoclast fusion by membrane-cortex attachment and BAR proteinsJ Cell BiolJuly 7, 2025Yumeng Wan, Yuri L. Nemoto, Tsukasa Oikawa, Kazunori Takano, Takahiro K. Fujiwara, Kazuya Tsujita, Toshiki ItohBioLegend, and MG132 (474790) from Merck Millipore. Recombinant mouse RANKL was purchased from R&D Systems (462-TEC-010) or prepared by affinity purification of bacterially expressed GST-RANKL (aa 158–316 of mouse RANKL),

(19) Injectable magnesium-bisphosphonate MOF-based bone adhesive prevents excessive fibrosis for osteoporotic fracture repairNature communicationsJuly 1, 2025Xiao T, Gong Z, Duan D, Yu H, Liu S, Jiang Y, Xing X, Wu Z, Wang L, Yang XB, et al.

(20) Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expressionScientific reportsJuly 1, 2025Jantarawong S, Khimmaktong W, Swangphon P, Lauterbach N, Nanakorn N, Panichayupakaranant P, Pengjam Y.

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TRANCE/TNFSF11/RANK L Protein

TRANCE/TNFSF11/RANK L Protein

TRANCE/TNFSF11/RANK L Protein

TRANCE/TNFSF11/RANK L Protein

TRANCE/TNFSF11/RANK L Protein

TRANCE/TNFSF11/RANK L Protein from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (157)

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TRANCE/TNFSF11/RANK L Protein from R&D Systems, a Bio-Techne Brand

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