MagneHis Protein Purification System from Promega

Product Specs
ItemMagneHis Protein Purification System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV8500
Quantity65 reactions
MethodMagnetic
Product TypeResins, Magnetic Beads
ModeAffinity Purification, Metal Chelate
Applicationspurification

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A novel MAP7D1 mutation causes mitotic defects and RPS14 accumulation in Shwachman−Diamond syndrome patient cellsDisease Models & MechanismsAugust 1, 2025Seren Kucukvardar, Arzu Karabaymutation of the microtubule-binding domain of MAP7D1 was examined using the pull-down method with the MagneHis™ Protein Purification System Kit (Promega, Madison, WI, USA) according to manufacturer's instructions. Briefly, 600 µg lysate obtained

(2) Design of cross-reactive antigens with machine learning and high-throughput experimental evaluationFrontiers in BioinformaticsJuly 16, 2025Chelsy Chesterman, Thomas Desautels, Luz-Jeannette Sierra, Kathryn T. Arrildt, Adam Zemla, Edmond Y. Lau, Shivshankar Sundaram, Jason Laliberte, Lynn Chen, Aaron Ruby, et al.(Millipore Sigma). Mutant fHbp samples were purified using one-step nickel-affinity chromatography using the MagneHis protein purification system (Promega) and automated with a Kingfisher Flex (ThermoFisher). Proteins were eluted in 50 mM Tris pH

(3) The PP2A‐B56 Binding Site LxxIxE Contributes to Asp‐Mediated Spindle Pole StabilityCytoskeleton (Hoboken)March 12, 2025Margaux Quiniou, Maria C. Burns, Aynsley McDermott, Karolina Jaworek, Stacey J. Scott, James G. Wakefield, Lori Borgalamylose resin, and eluted in column buffer with 20 mM maltose. His‐tagged Wdb was purified using the MagneHis Protein Purification System (Promega), washed on magnetic nickel beads, and then incubated with equal protein amounts of respective

(4) TRANSPARENT TESTA 16 collaborates with the MYB-bHLH-WD40 transcriptional complex to produce brown fiber cottonPlant PhysiologyDecember 2, 2024Yuanxue Li, Tian Yao, Chao Fu, Nian Wang, Zhiyong Xu, Ningyu Yang, Xianlong Zhang, Tianwang Wen, Zhongxu LinGST Protein Purification System (Promega V8603) and Magne His Protein Purification System (Promega V8550). The pull-down assays were performed as described previously (Yang et al. 2017). Y1H assays and LUC

(5) Cotton Bollworm (H. armigera) Effector PPI5 Targets FKBP17‐2 to Inhibit ER Immunity and JA/SA Responses, Enhancing Insect FeedingAdvanced ScienceNovember 1, 2024Yaxin Wang, Chuanying Zhu, Gefei Chen, Xuke Li, Mingjv Zhu, Muna Alariqi, Amjad Hussian, Weihua Ma, Keith Lindsey, Xianlong Zhang, et al.MagneGSTTM Protein Purification System (Promega V8603) and MagneHisTM Protein Purification System (Promega V8550). The pull‐down assay was performed as described previously.[ 106 ] The primers were listed in Table

(6) Evolution and subfunctionalization of CIPK6 homologous genes in regulating cotton drought resistanceNature CommunicationsJuly 9, 2024Weinan Sun, Linjie Xia, Jinwu Deng, Simin Sun, Dandan Yue, Jiaqi You, Maojun Wang, Shuangxia Jin, Longfu Zhu, Keith Lindsey, et al.samples were purified using the MagneGST Protein Purification System (Cat. #V8600, Promega) and the MagneHis Protein Purification System (Promega) according to the manufacturer’s instructions. The pull-down assay was performed similarly

(7) GhMYB52 Like: A Key Factor That Enhances Lint Yield by Negatively Regulating the Lignin Biosynthesis Pathway in Fibers of Upland Cotton (Gossypium hirsutum L.)International Journal of Molecular SciencesApril 30, 2024Yang Yang, Xue Zhou, Xi Zhu, Bo Ding, Linzhu Jiang, Huiming Zhang, Silu Li, Shuyan Cao, Mi Zhang, Yan Pei, et al.via centrifugation, resuspended in PBS, lysed via sonication, and purified using Ni magnetic beads (V8500, Promega). The eluted protein was desalted using ultrafiltration tubes, supplemented with a final concentration

(8) Antibody reactive immunomes of Ehrlichia chaffeensis and E. canis are diverse and defined by conformational antigenic determinantsFrontiers in Cellular and Infection MicrobiologyJanuary 9, 2024Tian Luo, Jignesh G. Patel, Xiaofeng Zhang, Jere W. McBrideand denatured proteins was also examined by dot immunoblot using IVTT-expressed proteins purified by MagneHis protein purification system (Promega, Madison, WI) according to the manufacturer. Immunoblots were probed with either HME or CME

(9) The GhMAP3K62-GhMKK16-GhMPK32 kinase cascade regulates drought tolerance by activating GhEDT1-mediated ABA accumulation in cottonJournal of Advanced ResearchSeptember 1, 2023Lin Chen, Bing Zhang, Linjie Xia, Dandan Yue, Bei Han, Weinan Sun, Fengjiao Wang, Keith Lindsey, Xianlong Zhang, Xiyan YangMagneGSTTM Protein Purification System (Promega V8603) and MagneHisTM Protein Purification System (Promega V8550). The pull-down assay was performed as described previously [39]. The primers are listed in Table S1. BiFC,

(10) Expression of Human β-defensin 2 (hBD-2) in Pichia Pastoris and Investigation of Its Binding Efficiency with ACE-2The Protein JournalAugust 1, 2023Şeymanur Çobanoğlu, Elif Arslan, Ayşenur Yazıcı, Serkan ÖrtücüqPCR Supermix (Solis BioDyne, Estonia) was used. The MagneHis™ Protein Purification System cat number V8500 was purchased from Promega Co. Ltd. (Madison, USA). ACE-2 protein (cat no: AVI10544) was utilized, and

(11) The role of Atg16 in autophagy, anthocyanin biosynthesis, and programmed cell death in leaves of the lace plant (Aponogeton madagascariensis)PloS oneJanuary 1, 2023Rowarth NM, Dauphinee AN, Lacroix CR, Gunawardena AHLAN.for 6 h at 37°C and the recombinant Atg16 were purified from cell-free extracts of E. coli with the MagneHis™ Protein Purification System (Promega). To test for antibody reactivity, 0.1 μg of recombinant lace plant Atg16 protein, 20 μg of

(12) Binding of the transcription factor MYC2-like to the ABRE of the OsCYP2 promoter enhances salt tolerance in Oryza sativaPLOS ONEOctober 14, 2022Hongbo Liu, Peng Cui, Bingxin Zhang, Jinbo Zhu, Cui Liu, Qingyang Licells, and the fusion protein was purified with a Magnehis Protein Purification Kit (Promega, Cat. No. V8500). The samples were analyzed by 12% SDS-PAGE and western blots with His tag antibody conjugated to horseradish

(13) A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS-CoV-2 Spike S1 RBD produced in Escherichia coliBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]September 1, 2022Conzentino MS, Gonçalves ACA, Paula NM, Rego FGM, Zanette DL, Aoki MN, Nardin JM, Huergo LF.previously with few modifications [18]. One milliliter of Ni2+ magnetic particles MagneHis (Promega cat V8550) was transferred to a 50-ml Falcon tube and washed twice with 1 ml of TBST 1X. The resin was resuspended

(14) Heat Shock Protein 70 in Penile Neurovascular Regeneration Requires Cystathionine Gamma-LyaseThe World Journal of Men's HealthMay 19, 2022Kalyan Ghatak, Guo Nan Yin, Soon-Sun Hong, Ju-Hee Kang, Jun-Kyu Suh, Ji-Kan RyuPull-down assay His-tag pull-down assays were performed according to instructions provided with the MagneHis Protein Purification System (Promega, Madison, WI, USA), with minor modifications. MCECs were treated with His-tagged recombinant

(15) Comparison of the potential activities of viral and bacterial chitinasesEgyptian Journal of Biological Pest ControlDecember 1, 2021Suleiman Abulikemu, Aydin Yesilyurt, Donus Gencer, Mehtap Usta, Remziye Nalcaciogluthe 6×His-tag at their N-terminal in E. coli BL21 (DE3) cells. Chitinase proteins were purified by using the MagneHis™ Protein Purification System Kit (Promega) and dialyzed for 24 h through 1 l of 1X PBS buffer, pH 7.5. The identification and purity

(16) Processing Technologies for Bee Products: An Overview of Recent Developments and PerspectivesFrontiers in NutritionNovember 3, 2021Xuan Luo, Yating Dong, Chen Gu, Xueli Zhang, Haile Ma(kDa),Separation/purification,Identification/analysis,References RJ,MRJP1 [A. melliferaMRJP (AmMRJP)]AmMRJPs 1–7,,"The MagneHis™ Protein Purification System (Promega, Madison, WI, USA)",SDS-PAGE and western blot analysis,"(19, 201)" RJ (China),MRJP1–3,228

(17) Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beadsAnalytical BiochemistryOctober 1, 2021Marcelo S. Conzentino, Tatielle P.C. Santos, Khaled A. Selim, Berenike Wagner, Janette T. Alford, Nelli Deobald, Nigela M. Paula, Fabiane G.M. Rego, Dalila L. Zanette, Mateus N. Aoki, et al.incubated for 5 min on ice with gentle mixing with 10 ml of Ni2+ magnetic particles (Promega cat number V8550) pre equilibrated in buffer 1. The beads were washed 2 times with 25 ml of buffer 1 and 2 times with

(18) Variable posttranslational modifications of severe acute respiratory syndrome coronavirus 2 nucleocapsid proteinGlycobiologySeptember 20, 2021Supekar NT, Shajahan A, Gleinich AS, Rouhani DS, Heiss C, Chapla DG, Moremen KW, Azadi P.1C). N(SP-) was purified from cell lysate using the MagneHis™ Protein Purification kit from Promega (V8500). The SDS PAGE showed that, in contrast to N(SP+), whose mass was higher due to glycosylation, N(SP-)

(19) Upregulation of Transferrin and Major Royal Jelly Proteins in the Spermathecal Fluid of Mated Honeybee (Apis mellifera) QueensInsectsJuly 31, 2021Hee-Geun Park, Bo-Yeon Kim, Jin-Myung Kim, Yong-Soo Choi, Hyung-Joo Yoon, Kwang-Sik Lee, Byung-Rae Jincultured in TC100 medium at 27 °C to produce the recombinant proteins, which were then purified using the MagneHis Protein Purification System (Promega, Madison, WI, USA). Protein concentrations were determined using the Bio-Rad Protein Assay

(20) LuxR Solos from Environmental Fluorescent PseudomonadsmSphereApril 28, 2021Cristina Bez, Sonia Covaceuszach, Iris Bertani, Kumari Sonal Choudhary, Vittorio Venturiin pETM-11 adding 100 mM of each AHL (C6-AHL, C8-AHL, and C14-AHL). Soluble fractions purified using MagneHis protein purification system (Promega Corp., Madison, WI, USA) is shown in the protein gel; Ppu16R was soluble when unbound to AHLs.

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