TNT SP6 Coupled Wheat Germ Extract System from Promega

Product Specs
ItemTNT SP6 Coupled Wheat Germ Extract System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL4130
Quantity40 reactions
Molecular Weight~74kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Compound Inflorescence (S) represses fruit growth and seed development in tomatoMolecular HorticultureJanuary 8, 2026Lin Weng, Li Wang, Enbai Zhou, Guixiang Wang, Xiaoyan Wang, Rong Li, Yuan Sun, Meng Li, Han Xiaoin vitro expression of Halo-S fusion protein using the TnT Wheat Germ Extract System (Promega, Cat # L4130). The protein was diluted in 25 μL 1 × PBS and incubated with library DNA for 1 h at room temperature

(2) Mechanism Analysis of Transcription Factor OsERF110 Regulating Rice Pollen Response to Heavy Ion IrradiationBiology (Basel)September 8, 2025Kai Sun, Jinzhao Liu, Jiameng Zhang, Haonan Li, Jian Zeng, Libin Zhou, Tao Guo, Chun ChenpFN19K HaloTag T7 SP6 Flexi vector, and express it using the TNT SP6 coupled wheat germ extraction system (Promega, Madison, WI, USA, L4130). Purify and capture the expressed proteins using Magne Halo Tag Beads (Promega, Madison, WI, USA, G7281).

(3) Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in XenopuseLifeDecember 12, 2024Mohamed El Amri, Abhay Pandit, Gerhard Schlosser,New England Biolabs,M0491S, Commercial assay or kit,TNT SP6 Coupled Wheat Germ Extract System,Promega,L4130, Commercial assay or kit,"Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye",Invitrogen,C10337, Commercial

(4) Gene regulatory network inference in soybean upon infection by Phytophthora sojaePLOS ONEJuly 7, 2023Brett Hale, Sandaruwan Ratnayake, Ashley Flory, Ravindu Wijeratne, Clarice Schmidt, Alison E. Robertson, Asela J. Wijeratneexpressed in vitro using the TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA, Cat #L4130) and purified using Magne HaloTag Beads (Cat #G7282). To prepare DAP-seq samples, hypocotyls were inoculated

(5) HSFA1a modulates plant heat stress responses and alters the 3D chromatin organization of enhancer-promoter interactionsNature CommunicationsJanuary 28, 2023Ying Huang, Jing An, Sanchari Sircar, Clara Bergis, Chloé Dias Lopes, Xiaoning He, Barbara Da Costa, Feng-Quan Tan, Jeremie Bazin, Javier Antunez-Sanchez, et al.expression vector and expressed using Promega TNT Coupled Wheat Germ Extract Systems (Promega, cat. no. L4130). The expressed proteins were immobilized on Magne HALO-Tag beads (Promega, G728A), washed, and incubated

(6) AR cooperates with SMAD4 to maintain skeletal muscle homeostasisActa neuropathologicaJune 1, 2022Forouhan M, Lim WF, Zanetti-Domingues LC, Tynan CJ, Roberts TC, Malik B, Manzano R, Speciale AA, Ellerington R, Garcia-Guerra A, et al.translated into proteins using coupled in vitro transcription/translation TNT wheat germ (Promega, L4130) and E. coli S30 (Promega, L1030) extract systems following the manufacturer’s protocols, respectively.

(7) The B1 Domain of Streptococcal Protein G Serves as a Multi-Functional Tag for Recombinant Protein Production in PlantsFrontiers in Plant ScienceApril 25, 2022Shi-Jian Song, Hai-Ping Diao, Byeongho Moon, Areum Yun, Inhwan Hwanganalog m7G[5′]ppp[5′]G. The in vitro translation reaction mixture of wheat germ extracts (Promega, Cat. L4130) contained 10 mM creatine phosphate, 50 μg/ml creatine phosphokinase, 5 mM DTT, 2.1 mM magnesium acetate,

(8) Poxviruses and paramyxoviruses use a conserved mechanism of STAT1 antagonism to inhibit interferon signalingCell host & microbeMarch 9, 2022Talbot-Cooper C, Pantelejevs T, Shannon JP, Cherry CR, Au MT, Hyvönen M, Hickman HD, Smith GL.resin,IBA,2-4030-002 Anti-FLAG M2 affinity gel,Sigma-Alrich,A220 TnT coupled wheat germ extract (SP6),Promega,L4130 Lysing matric S (1/8”) metal beads,MPBio,116925100 Qiagen RNeasy mini kit,Qiagen,74104 RT2 First

(9) Knockdown of NIR Suppresses Breast Cancer Cell Proliferation via Promoting FOXO3OncoTargets and therapyJanuary 1, 2021Chen B, Dong C, Wang F, Wu J.proteins were transcribed and translated in vitro by using TNT® SP6 Coupled Wheat Germ Extract System (#L4130, Promega). The proteins were pretreated with Benzonase and RNase to degrade DNA and RNA. For each immunoprecipitation assay

(10) A human ESC-based screen identifies a role for the translated lncRNA LINC00261 in pancreatic endocrine differentiationeLifeAugust 3, 2020Gaertner B, van Heesch S, Schneider-Lunitz V, Schulz JF, Witte F, Blachut S, Nguyen S, Wong R, Matta I, Hübner N, et al.,Thermo Fisher Scientific,Cat# 23227, Commercial assay or kit,TnT Coupled Wheat Germ Extract System,Promega,Cat# L4130, "Chemical compound, drug",Penicillin-Streptomycin,Thermo Fisher Scientific,Cat# 15140122, "Chemical

(11) The carboxyl-terminal sequence of bim enables bax activation and killing of unprimed cellseLifeJanuary 24, 2020Xiaoke Chi, Dang Nguyen, James M Pemberton, Elizabeth J Osterlund, Qian Liu, Hetal Brahmbhatt, Zhi Zhang, Jialing Lin, Brian Leber, David W Andrewsand methods." Other,transcription/translation (TNT)-SP6 coupled wheat germ extract system,Promega,L4130,Used for synthesis of Bim and Bax proteins for chemical crosslinking Other,multi-purpose image scanner,Fuji

(12) HCF-1 Regulates De Novo Lipogenesis through a Nutrient-Sensitive Complex with ChREBPMolecular cellJuly 25, 2019Lane EA, Choi DW, Garcia-Haro L, Levine ZG, Tedoldi M, Walker S, Danial NN.HCF-1 plasmid and the TnT® Coupled Wheat Germ Extract System per manufacturer’s instructions (Promega, L4130). His-OGT WT or OGT D554N proteins were purified from E. Coli as previously described (Janetzko et al.,

(13) Convergent recruitment of TALE homeodomain life cycle regulators to direct sporophyte development in land plants and brown algaeeLifeJanuary 15, 2019Alok Arun et al.System Promega Promega:V8870 Commercial assay or kit TNT Coupled Wheat Germ Extract System Promega Promega:L4130 Commercial assay or kit ClarityTM chemiluminescent detection Biorad Biorad:1705060S Chemical compound,

(14) LAP2 Proteins Chaperone GLI1 Movement between the Lamina and Chromatin to Regulate TranscriptionCellJanuary 10, 2019Mirza AN, McKellar SA, Urman NM, Brown AS, Hollmig T, Aasi SZ, Oro AE.Reticulocyte In vitro translation kit,Promega,Cat # L2080 Wheat Germ In vitro translation kit,Promega,Cat # L4130 Deposited Data,Deposited Data,Deposited Data Mass Spectrometry BASU-labeling,This paper,Table S2–4 Experimental Models

(15) Mapping genome-wide transcription-factor binding sites using DAP-seqNature ProtocolsAugust 1, 2017Anna Bartlett, Ronan C O'Malley, Shao-shan Carol Huang, Mary Galli, Joseph R Nery, Andrea Gallavotti, Joseph R EckerPhusion High-Fidelity DNA Polymerase (NEB, cat. no. M0530) • TNT SP6 Coupled Wheat Germ Extract System (Promega, cat. no. L4130) or TNT SP6 Coupled Reticulocyte Lysate System (Promega, cat. no. L4600). Promega T7 systems can be

(16) A radioligand binding assay to measure anti-thyroperoxidase autoantibodies in miceJournal of Immunological MethodsApril 2, 2007Sarah L. Hayward, Kunimasa Suzuki, John F. Elliott

(17) Engineering Gene Networks to Emulate Drosophila Embryonic Pattern FormationPLoS BiologyFebruary 22, 2005Mark Isalan, Caroline Lemerle, Luis Serranoto 30 °C); and TNT Coupled Wheat Germ Extract System (Promega, Madison, Wisconsin, United States; #L4130 and #L4140), which comprised 20 μl of TNT wheat germ extract, 1.2 μl of TNT reaction buffer, 0.6 μl

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