SV 96 Total RNA Isolation System from Promega

Product Specs
ItemSV 96 Total RNA Isolation System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberZ3500
Time< 1 hr.
Quantity1 x 96 each
SourceCell/Tissue
Nucleic Acid TypeTotal RNA
Sample TypeTissue samples, cultured cells

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) RNA editing for the treatment of alpha-1 antitrypsin deficiencyNucleic Acids ResearchJanuary 14, 2026Prashant Monian, Chikdu Shivalila, Genliang Lu, Keith Bowman, Stearne Briem, Marissa Bylsma, Michael Byrne, Megan Cannon, Arindom Chatterjee, Morgan Dallaire, et al.initiation, cell lysates were collected by removing media and adding 100 µl of RNA lysis buffer (Promega, Z3500). Lysates were stored at −80°C until further processing. Lysates were thawed at room temperature and

(2) Impact of stereopure chimeric backbone chemistries on the potency and durability of gene silencing by RNA interferenceNucleic Acids ResearchMay 22, 2023Wei Liu, Naoki Iwamoto, Subramanian Marappan, Khoa Luu, Snehlata Tripathi, Erin Purcell-Estabrook, Juili Dilip Shelke, Himali Shah, Anthony Lamattina, Qianli Pan, et al.48 h, and total RNA was extracted and purified using SV96 Total RNA Isolation kit (Promega, Cat. No. Z3505), and eluted with 100 μl of nuclease-free H2O. cDNA was produced with 5 μl of RNA sample in a total

(3) Control of backbone chemistry and chirality boost oligonucleotide splice switching activityNucleic Acids ResearchJune 10, 2022Pachamuthu Kandasamy et al.extracted from human or mouse myoblasts using Promega SV96 Total RNA isolation kits (Promega, Cat. No. Z3500). Briefly, lysate (in Trizol) was transferred to a 96-well plate, extracted with chloroform for 10 min

(4) Transcriptional correlates of malaria in RTS,S/AS01-vaccinated African children: a matched case-control studyeLifeJanuary 21, 2022Moncunill G, Carnes J, Chad Young W, Carpp L, De Rosa S, Campo JJ, Nhabomba A, Mpina M, Jairoce C, Finak G, et al.Seattle Children's Research Institute ( Seattle) using the Promega SV96 Total RNA Isolation kit (Cat# Z3500, Promega) following the manufacturer’s protocol. Samples kept in RNAprotect were centrifuged at 4000

(5) Single-cell RNA-seq reveals transcriptomic heterogeneity mediated by host-pathogen dynamics in lymphoblastoid cell lineseLifeJanuary 27, 2021SoRelle ED, Dai J, Bonglack EN, Heckenberg EM, Zhou JY, Giamberardino SN, Bailey JA, Gregory SG, Chan C, Luftig MA.primers CACCAGTG TGGCCTTG TTGGCTTG Commercial assay or kit SV96 Total RNA Isolation Kit Promega Cat #Z3500 Commercial assay or kit High-Capacity cDNA Reverse Transcription Kit Thermo Cat #4368814 Commercial assay

(6) The role of C16:0 ceramide in the development of obesity and type 2 diabetes: CerS6 inhibition as a novel therapeutic approachMolecular MetabolismMarch 1, 2019Suryaprakash Raichur, Bodo Brunner, Maximilian Bielohuby, Gitte Hansen, Anja Pfenninger, Bing Wang, Jens C. Bruning, Philip Just Larsen, Norbert Tennagelsincubated for 72 h at 37 °C. RNA extraction was done using the SV96 Total RNA Isolation System (Promega #Z3505) according to the manufacturer's protocol including a DNase step during the procedure. For cDNA synthesis

(7) Niraparib activates interferon signaling and potentiates anti-PD-1 antibody efficacy in tumor modelsScientific reportsFebruary 12, 2019Wang Z, Sun K, Xiao Y, Feng B, Mikule K, Ma X, Feng N, Vellano CP, Federico L, Marszalek JR, et al.1% β-mercaptoethanol, followed by mRNA purification using Promega’s SV96 Total RNA Isolation System (Z3500) according to the manufacturer’s instructions (Promega, Madison, WI). The expression of IFNB1 (TaqMan

(8) Replication Study: Systematic identification of genomic markers of drug sensitivity in cancer cellseLifeJanuary 9, 2018John P Vanden Heuvel, Ewa Maddox, Samar W Maalouf, Elizabeth Iorns, Rachel Tsui, Alexandria Denis, Nicole Perfito, Timothy M Errington, Reproducibility Project: Cancer Biology(Promega, cat# Z3051). RNA extraction was performed with the SV96 Total RNA Isolation System (Promega, cat# Z3505) according to manufacturer’s instructions. RNA preps were eluted in 100 µl nuclease free water (Promega,

(9) Low-Dose Memantine Attenuated Morphine Addictive Behavior Through its Anti-Inflammation and Neurotrophic Effects in RatsJournal of Neuroimmune PharmacologyJune 1, 2012Shiou-Lan Chen, Pao-Luh Tao, Chun-Hsien Chu, Shih-Heng Chen, Hsiang-En Wu, Leon F. Tseng, Jau-Shyong Hong, Ru-Band Lureaction (QPCR). Total RNA was extracted from each tissue sample using a total RNA isolation kit (SV 96, Z3500; Promega, Madison, WI, USA) and synthesized into first-strand cDNA using a high-efficacy cDNA synthesis

(10) Kinome-wide functional genomics screen reveals a novel mechanism of TNFα-induced nuclear accumulation of the HIF-1α transcription factor in cancer cellsPloS oneJanuary 1, 2012Schoolmeesters A, Brown DD, Fedorov Y.well. Gene expression analysis The SV 96 Total RNA Isolation System (Promega, Madison, WI, catalog #Z3505) was used for total RNA purification. The Nanodrop (Thermo Fisher Scientific) was used to determine

(11) Selective killing of mixed lineage leukemia cells by a potent small-molecule DOT1L inhibitorCancer cellJuly 12, 2011Daigle SR, Olhava EJ, Therkelsen CA, Majer CR, Sneeringer CJ, Song J, Johnston LD, Scott MP, Smith JJ, Xiao Y, et al.concentration response experiments. RNA from cells was isolated using the 96-well Promega RNA isolation kit (Z3505) according to the manufacturer’s protocol. Approximately 30% of the total RNA isolated was reverse transcribed

(12) Double-stranded regions are essential design components of potent inhibitors of RISC functionRNAMay 1, 2007Annaleen Vermeulen, Barbara Robertson, Andrew B Dalby, William S Marshall, Jon Karpilow, Devin Leake, Anastasia Khvorova, Scott Baskervillewas extracted from transfected cells using the SV 96 Total RNA Isolation System (Promega, Catalog No. Z3505). The entire extract was then used for the determination of mRNA levels by branched DNA assay (QuantiGene

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