RNasin Ribonuclease Inhibitor from Promega

Product Specs
ItemRNasin Ribonuclease Inhibitor
CompanyPromega
Price Get Pricing Get Quote for this Product
Catalog NumberN2115
Quantity10,000u
Concentration20 - 40 U/µL
SourceHuman Placenta

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Single‐Cell Transcriptomic Atlases of Camels and Cattle Unravel Molecular Evolution of Digestive and Metabolic SystemsAdvanced ScienceFebruary 3, 2026Tao Shi, Huiquan Shan, Haoping Wang, Xi Guo, Houcheng Li, Bo Han, Senlin Zhu, Fei Wang, Guanghui Tan, Zhannur Niyazbekova, et al.(Ambion), 10 mg/mL bovine serum albumin (Ambion), 5 mM MgCl2 (Ambion), 0.12 U/µL RNasin Plus (Promega, #N2115), and 1 × Complete Protease Inhibitor Cocktail (Roche, #11697498001). The tissue underwent two additional

(2) A role for the cholinergic neuron circadian clock in RNA metabolism and mediating neurodegenerationLife Sci AllianceDecember 15, 2025Sharon B Tam, Nathan J Waldeck, Matthew Wright, Jelena Mojsilovic-Petrovic, Erin M Baker, Evangelos Kiskinis, Joseph Bass, Robert G Kalb12 mM MgCl2, 1% Tergitol (NP-40), 1 mM dithiothreitol (DTT), 200 U/ml RNasin Ribonuclease Inhibitor (N2115; Promega), 1 mg/ml heparin, 100 μg/ml cycloheximide, and Protease Inhibitor Cocktail (P8340; Sigma-Aldrich)

(3) Analyzing the Translatome of Lymphatic and Venous Endothelial Cells In Vivo via Translating Ribosome Affinity Purification (TRAP)BIO-PROTOCOLDecember 5, 2025Olamide Olayinka, Leia Zarinebaf, Hyun Min Jung23200) 6. 1 mM DTT (Sigma, catalog number: 646563) 7. 200 units/mL RNAsin (Promega, catalog number: N2115) 8. 1 mg/mL heparin (Sigma, catalog number: H3393-10KU) 9. 10% NP-40 (Roche, catalog number: 11-332-473-001) 10.

(4) In vivo profiling of the endothelium using 'AngioTag' zebrafishAngiogenesisJuly 4, 2025Mayumi F. Miller, Leah J. Greenspan, Derek E. Gildea, Kathryn Monzo, Gennady Margolin, Van N. Pham, Keith K. Ameyaw, Lisa Price, Natalie Aloi, Amber N. Stratman, et al.(Sigma, Cat. #646563), 1 × Protease inhibitors (Sigma, Cat. #P8340), 200 units/mL RNAsin (Promega, Cat. #N2115), 100ug/mL Cycloheximide (Sigma Cat. #7698), 1mg/mL Heparin (Sigma, Cat. #H3393-10KU). Lysates were

(5) RiboTag-based RNA profiling uncovers oligodendroglial lineage-specific inflammation in autoimmune encephalomyelitis: implications for pathogenesisJ NeuroinflammationMay 21, 2025Yuhang Wang, Sudeep Ghimire, Ashutosh Mangalam, Zizhen Kang100 µg/ml cycloheximide, a protease inhibitor cocktail, 1 mg/ml heparin, RNase inhibitors (Promega, #N2115), and 1 mM dithiothreitol (DTT).The resulting homogenates underwent centrifugation, and the supernatant

(6) Mycobacterium tuberculosis modulates NUDT21-mediated alternative polyadenylation to enhance FTH1 expression in macrophages and promotes intracellular growthFront Cell Infect MicrobiolMay 2, 2025Xiaoqian Liu, Ningjian Cai, Youchao Dai, Xinchun Chen, Xiaobin Zeng1% TritonX-100, 1 mM DTT) supplemented with protease inhibitors and an RNase inhibitor (Promega, cat#N2115). The lysate was centrifuged, and the supernatant was collected. Protein concentrations in the lysate

(7) Herpes simplex virus 1 encodes a STING antagonist that can be therapeutically targetedCell Reports MedicineApril 15, 2025An Wang, Qianqian Peng, Huidi Fan, Wenting Ji, Jing Lou, Xi Zhou, Yujie RenEN0521 Protease inhibitor cocktail,MCE,Cat# HY-K0010 ECL,4A Biotech,Cat# BN01036 RNase inhibitor,Promega,Cat# N2115 Protein A/G Magnetic Beads,MCE,Cat# HY-K0202 Streptavidin Magnetic beads,MCE,Cat# 563175 qPCR SYBR

(8) Examination of the functions and mechanism of KCP in mediating paclitaxel resistance in cervical squamous carcinoma cellsFront OncolApril 14, 2025Yue He, Jian-Qing Xu, Jing-Jing Zhang, Zhen-You Liu, Chen Ji, Yang Liu, Yun-Fan Wang, Ming Wang, Yu-Mei Wu, Yan WangPu Fei Company, 3101-100), M-mlV (Promega, M1705), dNTPs (Promega, U1240), RNase Inhibitor (Promega, N2115), oligo dT (Shanghai Biotech Company, B0205), Bulge-Loop™ miRNA qPCR Primer Set (Guangzhou Ruibo Company),

(9) Increased Myocardial MARK4 Expression in Patients with Heart Failure and Sleep-Disordered BreathingInt J Mol SciApril 11, 2025Bettina Seydel, Philipp Hegner, Anna-Maria Lauerer, Sönke Schildt, Fatma Bayram, Maria Tafelmeier, Dominik Wermers, Leopold Rupprecht, Christof Schmid, Stefan Wagner, et al.PCR nucleotide mix (Promega, catalog number C1145), RNasin® ribonuclease inhibitor (Promega, catalog number N2115), reverse transcriptase (Promega, catalog number M170B), and reverse transcriptase 5x reaction buffer

(10) Post-mortem interval leads to loss of disease-specific signatures in brain tissueeNeuroFebruary 27, 2025Kimberly C. Olney, Katelin A. Gibson, Mika P. Cadiz, Negin Rahimzadeh, Vivek Swarup, John D. Fryer(Miltenyi Biotec, #130-128-024), followed by Miltenyi debris removal. Promega RNase Inhibitor (Promega, #PR-N2115) was included in all buffers and wash steps at a final concentration of 0.2 U/µl to prevent RNA degradation.

(11) Revealing the Transcriptional and Metabolic Characteristics of Sebocytes Based on the Donkey Cell Transcriptome AtlasAdvanced ScienceFebruary 27, 2025Yu Tian, Shuqin Liu, Hongtao Shi, Jianjun Li, Xinglong Wan, Yujiang Sun, Huayun Li, Ning Cao, Zhixi Feng, Teng Zhang, et al.sucrose (Ambion), 10 mg mL−1 BSA (Ambion), 5 × 10−3 m MgCl2 (Ambion), 0.12 U µL−1 RNasin Plus (Promega, N2115), and 1× cOmplete Protease Inhibitor Cocktail (Roche, 11697498001). The tissue was then homogenized

(12) Myocardial DYRK1B Expression Is Increased in Patients with Impaired Cardiac Contractility and Sleep-Disordered BreathingAntioxidants (Basel)January 29, 2025Fatma Bayram, Philipp Hegner, Anna-Maria Lauerer, Sönke Schildt, Dominik Wermers, Maria Johanna Baier, Julian Mustroph, Maria Tafelmeier, Zdenek Provaznik, Christof Schmid, et al.(Promega, Madison, WI, USA, catalog number C1181), RNasin® ribonuclease inhibitor (Promega, catalog number N2115), reverse transcriptase (Promega, catalog number M170B), reverse transcriptase 5x reaction buffer (Promega,

(13) Single-cell transcriptome atlas of lamprey exploring Natterin- induced white adipose tissue browningNat CommunJanuary 17, 2025Yue Pang, Yating Qin, Zeyu Du, Qun Liu, Jin Zhang, Kai Han, Jiali Lu, Zengbao Yuan, Jun Li, Shanshan Pan, et al.(Invitrogen, 15503022), 10 mg /ml BSA (SIGMA, A1933), 5 mM MgCl2 (SIGMA, 63069), 0.12 U/μl RNasein (Promega, N2115) and 1× cOmplete Protease Inhibitor Cocktail (Roche, 11697498001) in 2 ml Dounce homogenizer (KIMBLE,

(14) RiboTag-based RNA-Seq uncovers oligodendroglial lineage-specific inflammation in autoimmune encephalomyelitisbioRxivDecember 28, 2024Yuhang Wang, Sudeep Ghimire, Ashutosh Mangalam, Zizhen Kangμg/ml cycloheximide, a protease inhibitor cocktail, 1 mg/ml heparin, RNase inhibitors (Promega, Cat#N2115), and 1 mM dithiothreitol (DTT). The resulting homogenates underwent centrifugation, and the supernatant

(15) Integrating fragment-based screening with targeted protein degradation and genetic rescue to explore eIF4E functionNat CommunNovember 20, 2024Swee Y. Sharp, Marianna Martella, Sabrina D'Agostino, Christopher I. Milton, George Ward, Andrew J. Woodhead, Caroline J. Richardson, Maria G. Carr, Elisabetta Chiarparin, Benjamin D. Cons, et al.(Starlab, cat#I1402-9700). 22.5 µl IVT reaction mix (1.5 mL HeLa lysate, 60 µl RNAsin (Promega, cat#N2115), 300 µl accessory proteins, 600 µl reaction buffer, 200 µl nuclease free water and 30 µl 1ug/ml mRNA)

(16) Microglia are not necessary for maintenance of blood-brain barrier properties in health, but PLX5622 alters brain endothelial cholesterol metabolismNeuronSeptember 4, 2024Caterina P. Profaci, Sean S. Harvey, Kaja Bajc, Tony Z. Zhang, Danielle A. Jeffrey, Alexander Z. Zhang, Kelsey M. Nemec, Hayk Davtyan, Carleigh A. O’Brien, Gabriel L. McKinsey, et al.(irradiated)","Research Diets, Inc.",Cat. # D15111301i RNasin Ribonuclease Inhibitor,Promega,Cat. # N2115 Cycloheximide,Sigma Aldrich,Cat. # C7698 DL-Dithiothreitol solution,Sigma Aldrich,Cat. # 646563 Pierce Protein G Magnetic Beads

(17) Opto-seq reveals input-specific immediate-early gene induction in ventral tegmental area cell typesNeuronAugust 21, 2024Rhiana C. Simon, Mary C. Loveless, Joshua X. Yee, Brandon Goh, Su G. Cho, Zainab Nasir, Koichi Hashikawa, Garret D. Stuber, Larry S. Zweifel, Marta E. Sodengradient medium,Sigma,Cat#D1556 Protease Inhibitor Cocktail,Sigma,Cat#P8340 RNAse Inhibitor,Promega,Cat#N2115 Vectashield Vibrance antifade mounting medium,Vector Laboratories,Cat#H-1700-10 Critical commercial

(18) Multimodal cell atlas of the ageing human skeletal muscleNatureMay 1, 2024Lai Y, Ramírez-Pardo I, Isern J, An J, Perdiguero E, Serrano AL, Li J, García-Domínguez E, Segalés J, Guo P, et al.containing 250 mM sucrose (Sigma-Aldrich, S8501), 10 mg ml–1 BSA, 5 mM MgCl2, 0.12 U μl–1 RNasin (Promega, N2115) and 1× cOmplete Protease Inhibitor Cocktail (Roche, 11697498001). Frozen tissues were kept in an ice

(19) Inhibition of RIPK1 alleviating vascular smooth muscle cells osteogenic transdifferentiation via Runx2iScienceFebruary 16, 2024Li Y, Jie W, Qi Y, Mo M, Lian Y, Yin L, Huang H.,Thermo Fisher Scientific,Cat:#11668019 Lipofectamine RNAiMAX,Cat:#11668019,Cat:#13778150 DNase,Takara,Cat:#2270A RNasin,Promega,Cat:#N2115 Recombinant RNase inhibitor,Takara,Cat:#2313A RNase A,ThermoFishe,Cat:#EN0531 Prime Script RT reagent

(20) Lateral hypothalamic glutamatergic inputs to VTA glutamatergic neurons mediate prioritization of innate defensive behavior over feedingNature CommunicationsJanuary 9, 2024M. Flavia Barbano, Shiliang Zhang, Emma Chen, Orlando Espinoza, Uzma Mohammad, Yocasta Alvarez-Bagnarol, Bing Liu, Suyun Hahn, Marisela Moralesfor 2 h at 30 °C in DEPC-treated PB supplemented with 4% BSA, 0.3% Triton X-100, and RNasin (5 μl/ml, N2115, Promega, Madison, WI, USA). After rinsing with DEPC-PB, sections were incubated with secondary donkey

Be the First to Write a Review!