PURExpressRibosome Kit from New England Biolabs

Product Specs
ItemPURExpressRibosome Kit
CompanyNew England Biolabs
Price $374.00 Inquire
Catalog NumberE3313S
Quantity10 reactions
SystemPURE system technology
ApplicationsProteomics & Glycomics
Temperature-80 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Dual site targeting of the bacterial 70S ribosome by tetracyclinesNat CommunMay 19, 2026Swapnil C. Devarkar, Ivan B. Lomakin, Jimin Wang, Ayman Grada, Christopher G. Bunickribosomes from E. coli were derived from PURExpress® Δ Ribosome Kit (New England Biolabs, USA. Cat. # E3313S) and used according to the manufacturer’s protocol. Preparation of the reassociated 70S ribosomes After

(2) Sequence-specific trapping of EF-Tu/glycyl-tRNA complex on the ribosome by bottromycinbioRxivMarch 16, 2026Dmitrii Y. Travin, Ritwika S. Basu, Madhura N. Paranjpe, Dorota Klepacki, Anna I. Zhurakovskaya, Nora Vázquez-Laslop, Alexander S. Mankin, Yury S. Polikanov, Matthieu G. Gagnonin vitro translation reaction was run using the PURExpress® in vitro Protein Synthesis ΔRibosome kit (Cat. No. E3313S, NEB) supplemented with purified E. coli MRE600 70S ribosomes. The mRNA template was in vitro transcribed

(3) Structural modification of oxazolidinone antibiotics alters nascent peptide stalling preference and peptide trajectory through the ribosomebioRxivFebruary 21, 2026Jordan I. Kleinman, Tushar Raskar, Dorota Klepacki, Teresa Szal, Nora Vázquez-Laslop, Alexander S. Mankin, James S. Fraser, Danica Galonić Fujimoristudies by in vitro transcription/translation. 100 μL reactions composed of PURExpress Δribosome kit (NEB E3313S) with 0.8 U/uL SUPERase-In, 100 ng DNA template, 240 pmol purified MRE600 ribosomes, and 5000 pmol (20x

(4) Trigger factor accelerates nascent chain compaction and foldingProceedings of the National Academy of SciencesJuly 29, 2025Katharina Till, Anne-Bart Seinen, Florian Wruck, Vanda Sunderlikova, Carla V. Galmozzi, Alexandros Katranidis, Bernd Bukau, Günter Kramer, Sander J. Tansribosomes of the cell-free transcription/translation mix of the PURE system (67) (New England BioLabs, E3313S) is used to synthesize the DHFR constructs. The system was supplemented with 10 μM of modified tRNA

(5) Arrest Peptide Profiling resolves co-translational folding pathways and chaperone interactions in vivoNat CommunJuly 24, 2025Xiuqi Chen, Vincent J. Hilser, Christian M. Kaisercodon. Ribosomes with a SpyTag-labeled L17 subunit was used during the in vitro translation system (NEB, E3313S) to generate the stalled RNC. The reaction products were coupled to SpyCatcher conjugated double-stranded

(6) Transient disome complex formation in native polysomes during ongoing protein synthesis captured by cryo-EMNature CommunicationsFebruary 26, 2024Timo Flügel, Magdalena Schacherl, Anett Unbehaun, Birgit Schroeer, Marylena Dabrowski, Jörg Bürger, Thorsten Mielke, Thiemo Sprink, Christoph A. Diebolder, Yollete V. Guillén Schlippe, et al.assays Translations were performed in the PURE translation system using the PURExpress delta ribosome kit (NEB, #E3313S) according to the manufacturer’s protocol with a few modifications. Translation reactions were supplemented

(7) AP profiling resolves co-translational folding pathway and chaperone interactions in vivobioRxivSeptember 8, 2023Xiuqi Chen, Christian M. Kaisercodon. Ribosomes with a spytag labeled L17 subunit was used during in vitro translation system (NEB, E3313S) to generate the stalled RNC. The reaction products were supplemented with spycatcher conjugated double

(8) Sarecycline inhibits protein translation in Cutibacterium acnes 70S ribosome using a two-site mechanismNucleic Acids ResearchApril 11, 2023Ivan B Lomakin, Swapnil C Devarkar, Shivali Patel, Ayman Grada, Christopher G Bunickin the PURExpress® Δ Ribosome Cell-free Protein Synthesis System (New England Biolabs, USA, cat. # E3313S). Cell-free extract was prepared according to manufacturer instructions. Purified 70S ribosomes from

(9) Structural basis for context-specific inhibition of translation by oxazolidinone antibioticsNature Structural & Molecular BiologyFebruary 14, 2022Kaitlyn Tsai, Vanja Stojković, D. John Lee, Iris D. Young, Teresa Szal, Dorota Klepacki, Nora Vázquez-Laslop, Alexander S. Mankin, James S. Fraser, Danica Galonić Fujimoriin vitro transcription–translation. Reactions (100-μl volume) were prepared using the PURExpress △ Ribosome Kit (New England Biolabs, E3313S) containing 0.8 U μl−1 of SuperASE-In, ~1,100ng of DNA template encoding the stalling peptide sequence

(10) The ribosome modulates folding inside the ribosomal exit tunnelCommunications BiologyDecember 1, 2021Florian Wruck, Pengfei Tian, Renuka Kudva, Robert B. Best, Gunnar von Heijne, Sander J. Tans, Alexandros Katranidisbiotinylated ribosomes was checked by synthesizing GFP emerald (GFPem) in vitro (PURExpress Δ ribosomes, NEB #E3313S, New England Biolabs) and measuring fluorescence of GFPem in a QM-7 spectrofluorometer (Photon Technology

(11) Defects in the Assembly of Ribosomes Selected for β-Amino Acid IncorporationBiochemistryNovember 12, 2019Fred R. Ward, Zoe L. Watson, Omer Ad, Alanna Schepartz, Jamie H. D. Catecarried out using the combination of PureExpress (ΔtRNA, Δaa (E6840S)) and PureExpress (Δribosome (E3313S)) kits (NEB) with the following modifications. To generate the fMVF-Flag WT peptide reactions contained

(12) The ribosomal protein S1-dependent standby site in tisB mRNA consists of a single-stranded region and a 5′ structure elementProceedings of the National Academy of SciencesAugust 6, 2019Cédric Romilly, Sebastian Deindl, E. Gerhart H. WagnerSI Appendix, SI Materials and Methods. To test 70SΔS1 functionality, the PURExpress ΔRibosome Kit (E3313S, New England BioLabs) was used instead and supplemented with purified 30S and 50S subunits. In Vitro

(13) The Ribosome Cooperates with a Chaperone to Guide Multi-domain Protein FoldingMolecular CellApril 1, 2019Kaixian Liu, Kevin Maciuba, Christian M. Kaiser,New England Biolabs,M0202 RNase A,ThermoFisher,Cat#EN0531 PURExpress ΔRibosome Kit,New England Biolabs,Cat#E3313S T7 MegaScript Kit,Ambion,Cat#AM1333 MEGAclear Kit,Ambion,Cat#AM1908 EDC,ThermoFisher,Cat#22980 Sulfo-NHS,ThermoFisher,Cat#24510 D-Biotin,ThermoFisher,Cat#B1595 Coenzyme A trilithium salt

(14) Translation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactionsNature CommunicationsDecember 1, 2018Thomas Philipp Hoernes et al.5′-CAATAGGATTACGTATTGCTACGTCCTCCTTGAAGTCGATG-3′). Prokaryotic in vitro translation In vitro translation (IVT) employing the PURExpress Δ ribosome system (NEB, E3313S) was performed as described by the manufacturer33,65. Briefly, 1 µM mRNAs were translated in the presence

(15) Multiple prebiotic metals mediate translationProceedings of the National Academy of SciencesNovember 27, 2018Marcus S. Bray, Timothy K. Lenz, Jay William Haynes, Jessica C. Bowman, Anton S. Petrov, Amit R. Reddi, Nicholas V. Hud, Loren Dean Williams, Jennifer B. Glasspolymerase, and transcription/translation factors in 10 mM Mg2+) from the PURExpress Δ Ribosome Kit (E3313S; New England Biolabs), 0.1 mM amino acid mix (catalog # L4461; Promega), Bray et al. and 0.2 mM tRNAs

(16) The shape of the bacterial ribosome exit tunnel affects cotranslational protein foldingeLifeNovember 26, 2018Renuka Kudva, Pengfei Tian, Fátima Pardo-Avila, Marta Carroni, Robert B Best, Harris D Bernstein, Gunnar von Heijnemethods Enzymes and chemicals The PURExpress Δ Ribosome kit was purchased from New England Biolabs (Cat no. E3313S). The components used to prepare Lysogeny Broth (LB Medium) for ribosome isolation were obtained from

(17) The shape of the bacterial ribosome exit tunnel affects cotranslational protein foldingElifeNovember 26, 2018Renuka Kudva, Pengfei Tian, Fátima Pardo-Avila, Marta Carroni, Robert B Best, Harris D Bernstein, Gunnar von Heijnemethods Enzymes and chemicals The PURExpress Δ Ribosome kit was purchased from New England Biolabs (Cat no. E3313S). The components used to prepare Lysogeny Broth (LB Medium) for ribosome isolation were obtained from

(18) A simple real-time assay for in vitro translationRNAFebruary 1, 2015Mark C. Capece, Guy L. Kornberg, Alexey Petrov, Joseph D. Puglisi(Lukavsky and Puglisi 2004). The cell-free protein synthesis system The PURExpress ΔRibosome kit (E3313S, New England Biolabs, Inc.) provided the basic materials for cell-free protein synthesis. In all translation

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