Titan One Tube RT-PCR Kit; For one-step Reverse Transcription-Polymerase Chain Reaction technique, utilizing the enzyme mixture of AMV Reverse Transcriptase and the Expand PCR High Fidelity enzyme blend
from
Roche Applied Science
Description
The Titan One Tube RT-PCR Kit provides reagents, including controls, control primers, nucleotides, RNase inhibitor, and PCR-grade water, for performing RT-PCR analysis. It is designed for quick, sensitive, and reliable analysis of RNA with high fidelity.
Specifications/Features
Kit Contents:
1. Titan™ enzyme mix One vial containing the Expand™ High Fidelity enzyme blend and AMV Reverse Transcriptase in storage buffer; 50 [micro]l 2. dNTP mix 10 mM mixture of dATP, dCTP, dGTP, and dTTP; 200 [micro]l, sodium salts 3. RNase inhibitor 5 U/[micro]l; 50 [micro]l 4. Human control RNA 2 pg/[micro]l; 50 [micro]l 5. Control primer mix 20 [micro]M; 20 [micro]l 6. Double-distilled water PCR-Grade 1 ml, 7. RT-PCR Reaction buffer 5x conc. (7.5 mM MgCl2 and DMSO); 1 ml. 8. DTT solution 100 mM; 1 ml 9. MgCl2 stock solution 25 mM; 1 ml
Comments:
For one-step Reverse Transcription-Polymerase Chain Reaction technique, utilizing the enzyme mixture of AMV Reverse Transcriptase and the Expand PCR High Fidelity enzyme blend.