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  • Human Granzyme B ELISA

    Human Granzyme B ELISA

    eBioscience
    Friday, May 01, 2015
    My research was to look at Granzyme B production in natural killer cells. Granzymes are serine proteases that are released by cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B ... read more
  • CellTiter-Blue for use in viability assays

    CellTiter-Blue for use in viability assays

    Promega
    Friday, May 01, 2015
    CellTiter-Blue was used to evaluate the viability/proliferation of U2OS cells (ATCC) treated with PLK1 or negative control siRNA to determine the appropriate positive control concentration to measure ... read more
  • Gel Extraction Kit

    Gel Extraction Kit

    Zymo Research
    Tuesday, April 28, 2015
    Making plasmids is an everyday challenge in research laboratories. A good gel extraction kit is critical for cleaning DNA inserts after restriction digestion or PCR from various resources. I have used... read more
  • It extracts DNA but it is not pure

    It extracts DNA but it is not pure

    QIAGEN - Sample & Assay Technologies
    Friday, April 24, 2015
    I used this Gel DNA recovery kit to extract DNA from agarose fragments that were subsequently used for cloning purposes. read more
  • Good phospho STAT6 Antibody

    Good phospho STAT6 Antibody

    Cell Signaling Technology
    Tuesday, April 21, 2015
    I was using the phospho-STAT6 antibody to determine if the IL-13 I purchased was causing activation of the JAK/STAT pathway in primary epithelial cells. This is a good antibody to detect the Tyr641 ... read more
  • Easy RNA clean up

    Easy RNA clean up

    QIAGEN - Sample & Assay Technologies
    Thursday, April 16, 2015
    Used this kit to clean up RNA from several sources: tissue, primary neurons, cell lines. The end goal is to run qPCR on these samples and cleaning up the RNA is the first step. The kit is easy to use ... read more
  • Cost effective and easy

    Cost effective and easy

    Thermo Scientific
    Wednesday, April 15, 2015
    Used this kit to convert to cDNA from primary neurons, cell lines, and DNA from tissues with the ultimate goal of running qPCR on the samples. The kit works very well and is relatively quick. The ... read more
  • Cotinine ELISA for mouse

    Cotinine ELISA for mouse

    Abnova Corporation
    Wednesday, April 15, 2015
    We measure cotinine levels in mouse plasma samples immediatley after exposure to E-cigarettes for 3 days (5Hr/day). Samples between control and E-cigarette exposure were used for comparison. read more
  • EPAC1 Western blot

    EPAC1 Western blot

    Cell Signaling Technology
    Tuesday, April 14, 2015
    Western blot of extracts of whole mouse brain was done using EPAC1 antibody. We wanted to see whether the protein was expressed in NHBE cells but I was only able to do a control which is the band in ... read more
  • NE-PER Nuclear and cytoplamic extraction reagent

    NE-PER Nuclear and cytoplamic extraction reagent

    Thermo Scientific Pierce Protein Research Products
    Friday, April 10, 2015
    This was used to isolate the nuclear extract from the cell lines in order to check the nuclear protein activity in compound treated cells. It was chosen for its ease of isolation and because it is ... read more
  • Very good HA antibody

    Very good HA antibody

    Cell Signaling Technology
    Thursday, April 09, 2015
    I used this anti-HA antibody to stain my N-terminal HA tagged NICD3 protein. NICD3 should primarily be in the nucleus and I see nice nuclear staining with the HA antibody. Overall, this is a good ... read more
  • Super signal west dura extended duration substrate for developing Western blots

    Super signal west dura extended duration substrate

    Thermo Scientific Pierce Protein Research Products
    Thursday, April 09, 2015
    This product was used to check the differential protein expression in proliferation and differentiation of neuronal cells involved in neuronal protection. This SuperSignal West Dura Extended Duration ... read more
  • Ready to use kit for glucose

    Ready to use kit for glucose

    Cayman Chemical
    Wednesday, April 08, 2015
    I am looking at the effect of eating yogurt before a meal on postprandial blood glucose change. Due to the large quantity of my samples, I chose the microplate method. This kit comes with all the ... read more
  • Easy to use for protein quantification

    Easy to use for protein quantification

    Thermo Scientific Pierce Protein Research Products
    Wednesday, April 08, 2015
    This product was used in Western blot assays looking at various proteins in the brain homogenate of MPTP-intoxicated animals. After isolating the region of interest, protein was purified, run on a gel... read more
  • Make sure you get the HS (high sensitive) kit

    Make sure you get the HS (high sensitive) kit

    R&D Systems
    Tuesday, April 07, 2015
    I am working on an intervention study to look at whether yogurt consumption can reduce chronic inflammation, which is linked to the development of various diseases. Il-6 has been used as biomarker for... read more
  • HER2 Antibody for Immunofluorescence/Western blot

    HER2 Antibody for Immunofluorescence/Western blot

    Cell Signaling Technology
    Monday, April 06, 2015
    Cell Signaling HER2 antibody #2242 was used for both Western blot and immunofluorescence microscopy to determine the level of HER2 silencing by siRNA. This antibody was previously used to detect HER2 ... read more
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