BD Biosciences
BD™ CD33 PerCP-Cy™5.5
341640
Clone P67.6 (ASR)
Info: Supplier Page
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CD33 is typically positive on monocytes (bright) and neutrophils (intermediate/dim) but completely negative on lymphocytes. We extensively use this antibody as one of the markers for acute myeloid leukemia specimens.
Flow cytometry
Blood and bone marrow
10-20 minutes @4°C
2% FBS
None
Blue (488nm) laser-equipped cytometer
Although PerCP-Cy™5.5 is a known "dim" fluorochrome, we were expecting better results from this antibody than what we got. The resolution of the three populations (monocytes, granulocytes, and lymphocytes) is just about enough to separate them. This is even weaker when multi-color assays are designed with this antibody.
N/A
Stains cells appropriately.
Dim expression on positive cells.
PerCP-Cy™5.5 should be reserved for markers that are highly expressed on the cells. Using it with CD33 probably does not work as well as needed.