Promega
Dual-Luciferase Reporter Assay System
E1980
Info:Supplier Page View Product Specs View Product
I used the dual luciferase assay to measure transcriptional activation using a Gal4 synthetic transcription assay. I co-transfected the GAL4 upstream activation sequence-driven firefly luciferase expression construct and the synthetic transcription factor constructs and measured firefly luciferase activity. The assay is straightforward to set up and all reagents required for the assay are supplied in the kit.
Measure luciferase activity using luminescence
Cells expressing various plasmids
Use 100 ul solutions for cells grown in 96-well plate
I measured luciferase activity to find out transcription activation domain of a transcription factor.
N/A
Easy to use.
The assay kit is expensive.
Good and reliable assay for measuring luciferase activity.