Rapid Assay From Promega for Detecting Lactate Secretion

January 25, 2024

State University of New York at Buffalo
Physiology and Biophysics
Postdoctoral Researcher

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Company:

Promega

Product Name:

Lactate-Glo assay kit

Catalog Number:

J5021

We measure cell culture lactate (secreted extracellular lactate) to assess energy metabolism changes, specifically glycolysis, in cultured immunomagnetically isolated astrocytes.

Experimental Design and Results Summary

Application

Metabolite assay

Starting Material

Cell culture medium

Tips

Requires luminescent plate reader.

Results Summary

We utilized Lactate-Glo assay kit from Promega to measure extracellular lactate secreted by cytochrome c oxidase (COX)-deficient astrocytes immunomagnetically isolated from adult mice (Cox10 KO mice). Cells were seeded at a density of 20,000 cells per well in 96-well plates precoated with poly-D-lysine. The next day, the medium was replaced with fetal bovine serum-free medium (Life Technologies, 11965092). After 3 hours of incubation, the medium was collected and centrifuged at 10,000 g for 5 min. The secreted L-lactate concentration in the supernatant was determined by measurement of luminescent signal.

DOI or PMID #

35275429

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Summary

The Good

Quick protocol ensures efficiency in experimental procedures, saving valuable time without compromising on the accuracy of results.

The Bad

Relatively high price.

The Bottom Line

The Lactate-Glo assay kit offers researchers consistent and reliable results, facilitated by its quick and well-explained step-by-step protocol. However, its notable drawback is the relatively high price, which may impact its accessibility for researchers operating within budget constraints.

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