Convenient to Use BaseScope Detection Reagent Kit From Advanced Cell Diagnostics

January 16, 2024

State University of New York at Buffalo
Physiology and Biophysics
Postdoctoral Researcher

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Quality of Results

Ease-of-Optimization

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Company:

Advanced Cell Diagnostics

Product Name:

BaseScope Detection Reagent v2 - RED

Catalog Number:

323900

We use BaseScope Detection Reagent v2 RED Kit to visualize single RNA molecules of interest (using custom BaseScope probes) in mouse-fixed frozen brain tissue.

Experimental Design and Results Summary

Application

In Situ Hybridization (ISH)

Starting Material

Mouse brain fixed frozen tissue

Tips

Staining intensity can be modified by adjusting the Amplification 7 time.

Results Summary

We use this kit combined with immunostaining to assess cell type-specific mRNA expression of the gene of interest. We follow manufacturer instructions (Technical Note MK 51-149/Rev B, ACD). Image: representative confocal image of dual BaseScope in situ hybridization for Npas3 mRNA (red punctate dots) and glial fibrillary acidic protein immunofluorescence (green). Section is counterstained with DAPI fluorescent dye to disclose tissue morphology. Image was obtained using a 63× objective.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

The kit offers straightforward instructions in the technical note and consistently delivers reliable and reproducible results.

The Bad

Cost per analysis is high.

The Bottom Line

The BaseScope kit stands out for its user-friendly design, offering straightforward instructions in the technical note that make experimentation seamless. The convenience of the reagent bottles enhances the overall user experience, providing easy access to the necessary components.One drawback is the limitation in the number of samples that can be analyzed per kit. Researchers may find the cost per analysis to be a limiting factor, especially when working with a large number of samples, impacting the overall cost-effectiveness of the kit for certain studies.

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