BioLegend's Fixable Kit for the Distinction of Live and Dead Cells

Leibniz Institute für Virologie
AG 61
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Zombie Aqua Fixable Viability Kit

Catalog Number:

423101

I used the Zombie Aqua Fixable Viability Kit in both human and mouse cells. The product works great and it is available in different fluorochromes (to be adapted to every FACS panel). The staining takes at least 30 minutes but allows a good separation of dead and alive cells also after fixation.

Experimental Design and Results Summary

Application

Immunology

Starting Material

Human and Mouse cells

Tips

It is very important to perform the staining in cells suspended in PBS. The staining takes 30-40 minutes in the dark at RT.

Results Summary

Good separation of dead cells from alive cells in different cells types (both human and mouse). The signal is bright and the staining can be performed before the extracellular staining.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy to use.

The Bad

Expensive.

The Bottom Line

Although a bit expensive,it is easy to use, fast and it allows a good recognition of dead cells at FACS. Recommended!

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