Versatile PCR Excellence: Unleashing the Power of the StepOnePlus Real-Time System

Houston Methodist Research Institute

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Performance

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

StepOnePlus Real-Time PCR System

Catalog Number:

4376600

To conduct mycoplasma testing using the StepOnePlus Real-Time PCR System, I employ specific primers targeting mycoplasma DNA and run quantitative PCR assays. The system's adaptability and precision enable accurate detection of mycoplasma contamination, ensuring reliable results for maintaining cell culture integrity.

Experimental Design and Results Summary

Application

PCR testing

Tips

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DOI or PMID #

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Summary

The Good

Leveraging the StepOnePlus Real-Time PCR System, I can confidently and precisely detect mycoplasma contamination in cell cultures through targeted DNA amplification with specific primers.

The Bad

The application takes an agonizingly long time to load, causing frustration and delays in getting started with tasks.

The Bottom Line

The StepOnePlus Real-Time PCR System stands out as a remarkably adaptable platform, proficiently catering to a broad spectrum of PCR applications. With its modular structure and capability to interface with different assay formats and chemistries, it proves ideal for tasks such as quantitative PCR, genotyping, gene expression analysis, and even digital PCR. This system grants researchers the versatility required to conduct a myriad of PCR experiments seamlessly within a singular instrument.

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