Merck
G418 Disulfate salt solution
G8168
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G418 stock was diluted to 250 mcg/ml in DMEM containing 10% FBS, 4 mM glutamine, 10 mM HEPES and puromycin (5 mcg/ml) and used to culture HEK293 cell line, stably expressing hRyR2-IRES-GFP.
Used to maintain stable gene expression.
Cells, culture medium
Stock solutions should be stored in aliquots at -20°C.
Cells expressing hRyR2 with GFP fluorescence and G418 resistance are maintained in the cell culture.
N/A
Easy to reconstitute in the culture medium.
The culture medium becomes slightly acidic.
G418 helps to maintain cell line having stable protein expression with G418 resistance.