Dependable Cell Dissociation Trypsin-EDTA Solution From Thermo Fisher

University of Tennessee HSC
College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher Scientific

Product Name:

Trypsin-EDTA (0.25%), phenol red

Catalog Number:

25200072

We routinely maintained various mammalian cell lines to test our hypothesis. I always use this trypsin-EDTA solution for dissociation and passaging of continuous cell lines.

Experimental Design and Results Summary

Application

Cell Culture

Starting Material

Mammalian cell lines

Tips

For longer shelf life prepare small aliquots and store frozen.

Results Summary

Within 3-5 minutes of treatment of adherent cells with this solution showed dissociation of cells and split them into more number of flasks to grow.

DOI or PMID #

N/A

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Summary

The Good

Very convenient to use for maintaining good cell lines.

The Bad

None.

The Bottom Line

Always prefer to use during cell culture experiments.

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