Highly Recommended Antibody From Cell Signaling for Western Blot

Babraham Institute

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

Tubulin antibody

Catalog Number:

2144

Clone Number:

Clean blots, sensitivity is good, great for blotting for tubulin as a loading control. They are used in MCF10a cells.

Experimental Design and Results Summary

Applications

Western blot

Sample

MCF10a cells

Primary Incubation

1:1000 in 5% BSA overnight at 4 degrees

Blocking Agent

5% BSA

Secondary Incubation

1:1000 in 5% BSA for 1 hour at room temperature

Tertiary Incubation

N/A

Detection

ECL reagent

Results Summary

Good antibody, lasts a while because of high sensitivity, so not too expensive. Great loading control for western blots.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Clean.

The Bad

None

The Bottom Line

Would recommend.

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