New England Biolabs
Q5® Hot Start High-Fidelity 2X Master Mix
M0494L
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I work with numerous strain variants of a bacterium that require verification once in a while. Some genes across the strains exhibit variations in both DNA sequence and length. I amplify these genes using Q5 DNA polymerase and common oligos and validate them through gel electrophoresis, followed by DNA sequencing.
Amplification of DNA and cloning.
N/A
While working with the Q5 DNA polymerase master mix, always store it in a mini freezer. Avoid placing it on ice.
As shown in the figure, I performed colony PCR and separated the amplified DNA using agarose gel electrophoresis. As expected, I observed significant differences and the absence of DNA, confirming the authenticity of my Enterococcus strains. Also, I noticed that colony PCR fails with other master mixes; however, with the Q5 master mix, colony PCR never fails.
Highly reliable when compared to other cheap master mixes.
Expensive.
The Q5 Master Mix (2X) from NEB provides a reliable solution for cloning purposes due to its outstanding quality. Strongly recommended.