cOmplete™ His-Tag Purification Resin for purifying His-tagged protein

July 29, 2023

Stanford University
Biochemistry
Postdoctoral Researcher

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Company:

COHISR-RO Roche

Product Name:

cOmplete™ His-Tag Purification Resin

Catalog Number:

5893682001

Info: Supplier Page 

I work with in-vitro protein phase separation assays which require high-quality purified proteins. The cOmplete™ His-Tag Purification Resin is a vital tool in protein purification for several reasons. Its nickel-chelating technology enables specific binding to the polyhistidine (His-tag) affinity tag, ensuring selective isolation of His-tagged proteins from complex mixtures. By exploiting the strong affinity of nickel for the His-tag, this resin offers an efficient and reliable method to achieve high-purity His-tagged proteins with minimal non-specific binding.

Experimental Design and Results Summary

Application

Protein purification

Starting Material

Cell lysates containing overexpressed His-tagged protein

Tips

Use low Imidazole for binding and washing steps. I do not go beyond 5 mM Imidazole.

Results Summary

The image displays the cOmplete™ His-Tag Purification Resin effectively binding to the GFP-tagged protein, demonstrating its specificity and affinity for the His-tag. This successful interaction confirms the resin's utility as a reliable tool for purifying His-tagged proteins.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

≥ 40 mg protein per ml bed volume of resin. The resin is compatible with 10mM EDTA, 10mM DTT during the purification (1 hour incubation), 6M guanidinium-HCl, 8M urea, pH 2.0 to pH 14.0.

The Bad

None.

The Bottom Line

cOmplete™ His-Tag Purification Resin was used to efficiently isolate His-tagged proteins with high specificity and yield.

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