Effective Ultrafilter for Extracellular Vesicle/Exosome Concentration and Staining

July 28, 2023

University of Toronto
Chemical/Biomedical Engineering
PhD Candidate

Overall

Performance

Ease-of-Optimization

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Company:

Millipore Sigma

Product Name:

Amicon Ultra-0.5 Centrifugal Filter Unit, 100kDa size cutoff

Catalog Number:

UFC510024

Info: Supplier Page 

Extracellular vesicles (EVs) isolated from cell culture media and resuspended in buffer were concentrated down to small volumes using 0.5ml ultrafilter units. The filter units also facilitated fluorescent staining and washing of EVs, allowing buffer and stains to pass through the membrane while retaining larger EVs.

Experimental Design and Results Summary

Application

Concentration and staining of extracellular vesicles.

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DOI or PMID #

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Summary

The Good

Simple and intuitive design, compatible with microcentrifuge tube sizes, and it is available in various size cut-offs for other applications such as protein concentration.

The Bad

Limited to 500 ul sample volume.

The Bottom Line

Great ultra filter system for staining EVs or concentrating proteins with small sample starting volumes.

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