Reliable TGN38 Antibody From Bio-Rad

Biophysics and Biochemistry
University of California, San Francisco
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

Sheep Anti-Rat TGN38

Catalog Number:

AHP499G

Clone Number:

N/A

Used for probing for a Golgi apparatus protein for fractionation experiments. The antibody is for rats but it works well for mice too. I'd rate it a 4/5 because it is a suitable antibody but not always the cleanest.

Experimental Design and Results Summary

Applications

Western blot

Sample

HL-1 cells

Primary Incubation

BSA, 1:2000

Blocking Agent

Milk

Secondary Incubation

Milk

Tertiary Incubation

N/A

Detection

HRP

Results Summary

Used this antibody to detect Golgi apparatus protein fractions in fractionation experiments using immortalized mouse cardiomyocytes HL-1.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Pretty reliable, can use for imaging too.

The Bad

Not much to complain, not always the most beautiful bands but gets you what you need.

The Bottom Line

Good Golgi antibody for rat/mouse cells.

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