Agilent Technologies
p53
M7001
DO-7
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This p53 antibody works well and I have used in both human tissues and human cell cultures. A specific marker that does not give a background and nonspecific staining.
Immunohistochemistry
HepG2 cell culture
1/100 dilution, diluent BSA %1, 2 hours at RT
%1 BSA
Biotinylated anti-mouse IgG
peroxidase conjugated streptavidin / AEC chromogen
ABC-peroxidase method
I get strong nuclear staining on neoplastic cells. Background is clear. You can easily count positive or negative cells.
N/A
Gill's hemalum as a background staining.
Countable nuclear positivity.
Nope
Agilent Technologies antibodies are expensive but as in the p53 they are without problems.