Good Phospho-p38 MAPK Antibody for Western Blot

Cardiovascular Biology
OMRF
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

p-p38MAPK

Catalog Number:

4511T

Clone Number:

N/A

The p38 signalling transduction pathway, a Mitogen-activated protein (MAP) kinase pathway, plays an essential role in regulating many cellular processes including inflammation, cell differentiation, cell growth and death.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse tissue and culture samples

Primary Incubation

1:1000 dilution overnight incubation

Blocking Agent

4% Skimmed milk

Secondary Incubation

1:10000 4% milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

Inflammation is a natural host defensive process that is largely regulated by macrophages during the innate immune response. Mitogen-activated protein kinases (MAPKs) are proline-directed serine and threonine protein kinases that regulate many physiological and pathophysiological cell responses.in our results angII stimulated cells time dependent manner increased phosphorylation of p38 levels.

DOI or PMID #

NA

Additional Notes

NA

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Summary

The Good

Great

The Bad

None

The Bottom Line

Good antibody referred to others.

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