Sensitive LDH Assay from BioLegend

Dana-Farber Cancer Institute
CIV
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

LDH-Cytox™ Assay Kit

Catalog Number:

426401

LDH-Cytox Assay™ Kit is a kit for determination of cytotoxicity by measuring lactate dehydrogenase activity released from damaged cells. LDH catalyzes dehydrogenation of lactate to pyruvate thereby reducing NAD+ to NADH. NADH reduces water-soluble tetrazolium salt in the presence of an electron mediator to produce an orange formazan dye.

Experimental Design and Results Summary

Application

Detection of c3ll lysis

Starting Material

N/A

Tips

N/A

Results Summary

This kit can show the help to calculate cytotoxity percentage. It's very sensitive. However, it's a little difeacult to follow the protocol

DOI or PMID #

N/A

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Summary

The Good

Sensitive.

The Bad

Protocol is a little difficult to follow.

The Bottom Line

In general, it is a good way to detect the cytotoxity of killing assay with NK cells or T cells.

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