Fast and Great Quality RNeasy Mini Kit for RNA Isolation

UCSF
BMS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

RNeasy Mini Kit

Catalog Number:

74106

The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the immune system– the mammalian defense to homeostatic alteration. Ketogenic diets (KDs) are extremely high in fat and very low in carbohydrates and have been shown to dramatically rewire whole-body metabolism. The KD-induced metabolic adaptation improves many physiological processes including aging, fatty acid utilization, and glucose homeostasis. However, the effects of KDs on host immunity remain less explored. Thus, the overall objective of my PhD studies will be to elucidate how KDs affect the immune response in adaptive and maladaptive contexts.

Experimental Design and Results Summary

Application

RNA isolation

Starting Material

Cells or Tissue

Tips

NA

Results Summary

The manufacturer protocol worked well and yielded great-quality of RNA from either cell culture or adipose tissue.

DOI or PMID #

NA

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Summary

The Good

Reliable results.

The Bad

Expensive

The Bottom Line

Highly recommended for high quality RNA isolation.

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