Antibody for Staining Mesenchymal Cells

December 22, 2022

OBGYN
University of Texas Medical Branch at Galveston
Graduate Student

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Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-vimentin

Catalog Number:

ab45939

Clone Number:

N/A

We are culturing mesenchymal cells in microfluidic devices. This antibody stains them well, with spindle shaped morphology clearly visible and brightly lit.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Cell culture

Primary Incubation

4 deg overnight

Blocking Agent

BSA

Secondary Incubation

Anti-Rb x Room temp x 1 hr

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Using anti-vimentin highlights the spindle shaped morphology of mesenchymal cells. The optimal dilution we've used is 1:300.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable and high quality.

The Bad

N/A

The Bottom Line

Antibody has great signal.

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