Excellent Collagenase Type 2

Houston Methodist Research Institute
Nanomedicine
Postdoctoral Researcher

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Quality of Results

Ease-of-Optimization

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Company:

Worthington Biochemical Corporation

Product Name:

Collagenase, Type 2

Catalog Number:

LS004174

We have used several bottles of this Type 2 collagenase for dissociation of tissues. Type 2 collagenase contains higher relative levels of protease activity, particularly clostripain, which is suited for the digestion of heart, bone, thyroid, cartilage, and liver tissues.

Experimental Design and Results Summary

Application

Tissue disassociation and cell isolation

Starting Material

Tissue

Tips

Every bottle has different activities. Need to calculate the amount base on the activity per gram.

Results Summary

We successfully got single cells from liver & lung tissues using type 2 collagenase. We tried flow cytometry and the cells didn't lose the marks. We also did cell culture and the cells grew very well.

DOI or PMID #

N/A

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Summary

The Good

Reliable

The Bad

Expensive

The Bottom Line

Expensive but great. Highly recommended.

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