Great Akt Antibody for Western Blotting

November 07, 2022

Babraham Institute

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

Akt Antibody

Catalog Number:

9272

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: AKT Antibodies

Used as a readout for PI3K signalling in MCF10a cells following 16 hours of starvation and then 15 minutes of EGF stimulation

Experimental Design and Results Summary

Applications

Western Blot

Sample

MCF10a

Primary Incubation

1:1000 in 5% BSA in TBST for 1 hour at room temperature

Blocking Agent

10% Milk in TBST

Secondary Incubation

1:2000 in 10% milk in TBST for 1 hour at room temperature

Tertiary Incubation

N/A

Detection

ECL western blotting reagent

Results Summary

This antibody was great for detecting Akt in MCF10a cells - it showed levels of total Akt were not changing despite observations that phosphorylated Akt was (following EGF stimulation).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very clear signal.

The Bad

Quite expensive.

The Bottom Line

Would definitely buy again.

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