Beckman Coulter
CD13-APC, Immu103.44, 0.5 mL, ASR
A87783
Immu103.44
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CD13 is expressed on granulocytes and monocytes (the myelomonocytic lineage). We opted for APC conjugate because it is typically bright. Although there are cheaper alternatives, we got this antibody from Beckman Coulter.
Flow Cytometry
Human whole blood (RBC lysed)
15 minutes at room temperature
1% bovine serum
N/A
Red laser (APC channel)
The results are shown in the figure: Granulocytes (pink) stained well. Monocytes are also positive. However, it was hard to resolve the monocytes from the lymphocytes. The use of side scatter helps. We would've liked it to be brighter, but the staining is acceptable.
Easy titration. No issue with compensation.
Relatively dim.
This antibody should work for most people, but if a strong signal is needed, then maybe try a different antibody/fluorochrome. Otherwise, it works OK.