Fisher Scientific
Gibco™ Trypsin-EDTA (0.05%), phenol red
25-300-120
Info:Supplier Page
I use it daily for routine cell culture passaging. The trypsin concentration I used the most is 0.05% for my cell type and experimental requirements. The best activity generally averages 37 degrees (incubator) for about 2-3 minutes. Stable even after multiple in and out of the refrigerator (4 degrees).
Routine cell culture passaging
Cell culture
The best activity generally averages 37 degrees (incubator) for about 2-3 minutes.
I daily use it for routine cell culture passaging at 0.05% for 2/3minutes in incubator. Also active at room temperature, but takes longer to act (about at least 5 minutes). The cells detach immediately and no loss of viability results (tested at the counter).
N/A
Not aggressive, resistant.
The best trypsin used in the lab because it is low-aggressive and resistant to multiple in/out from the refrigerator (4 degree).