Cell Signaling Technology
anti-p-S465S467-Smad2 antibody
3108
N/A
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BT20 cells were treated with 30 ng/ml TNFalpha for 30 minutes, and p-S465S467-Smad2 was examined by Western blot to check the response of TGFbeta signaling pathway. The results showed that this pathway was activated after TNFalpha treatment.
Western Blot
BT20
1/1000 dilution 4 degrees overnight
5% milk
1:1000 dilution
Room temperature 1 hour
ECL
BT20 cells were treated with 30 ng/ml TNFalpha for 30 minutes and p-S465S467-Smad2 was examined by Western blot to check the response of TGFbeta signaling pathway. The results showed that this pathway was activated after TNFalpha treatment.
The antibody works.
The antibody works well.