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APC/Fire™ 750 anti-mouse CD54 Antibody
116125
YN1/1.7.4
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment we are looking for markers of PC.
Flow Cytometry
Mouse spleen
Surface staining with CD54 APC-Fire750 (1/100), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer A5 Symphony BD
In this work, we were looking for surface markers that are differentially expressed by B cells (B220+) and plasma cells (PC). We found that CD54 is higher expressed by PC in comparison with B cells.
35464469
It works.
It is not very bright.
The antibody is good enough to be used.