Kit Didn't Work, but Likely User Error

Penn State College of Medicine
Neurosurgery
Graduate Student

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Company:

R&D Systems

Product Name:

TNF-alpha ELISA

Catalog Number:

DTA00D

Tried to use for media from astrocytes activated by amyloid beta. I did not detect any TNF-a, however, I discovered after the fact that astrocytes often do not secrete much TNF-a in response to amyloid beta (microglia are the primary source).

Experimental Design and Results Summary

Application

ELISA

Starting Material

Human astrocyte media

Tips

Don't use for astrocytes and amyloid beta.

Results Summary

Did not work, but likely my own fault.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Not sure

The Bad

Did not work for me.

The Bottom Line

Don't use for astrocytes and amyloid beta.

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