Great and Easy To Use for RT-qPCR

May 02, 2022

UCSF
Biomedical Sciences
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Thermo Fisher Scientific

Product Name:

Maxima Reverse Transcriptase

Catalog Number:

EP0741 / EP0742 / EP0743

I apply large-scale multi-omics assays in human samples for basic and translational biomedical research, from scRNA-Seq to epigenomics and genome-wide CRISPR screens. When not applying high-throughput technologies, I also conduct traditional molecular and cellular assays (e.g. RT-qPCR) at smaller scale (dozens to hundreds of samples). I was looking for a robust and simple-to-use RT enzyme mix to simplify my RT-qPCR workflow.

Experimental Design and Results Summary

Application

qRT-PCR

Starting Material

RNA

Tips

I always use the optional 30 minute extension just to be safe.

Results Summary

I’ve had no trouble obtaining excellent yields and qRT-PCR curves from a variety of different human RNA sample types. I typically use the same RNA input as other RT mixes I’ve used in the past, but those often required extra steps and somehow end up with worse Ct values when compared to Maxima.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Super easy to use, excellent yields, no optimization.

The Bad

No issues as of yet!

The Bottom Line

My lab has used the Transcriptor RT kit for over a decade, and after using Maxima, I’m planning on switching us fully over. If you do RT-qPCR routinely, this saves so much effort while producing excellent yields.

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