Perfect P-JNK Antibody for Western Blot

Cardiovascular Biology
OMRF
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell signaling

Product Name:

Phospho-SAPK/JNK

Catalog Number:

4671

Clone Number:

Info:Supplier Page View Product Specs

Browse Similar Products: JNK Antibodies

Angiotensin II increases blood pressure by a number of factors; the most important examples being vasoconstriction, sympathetic nervous stimulation, increased aldosterone biosynthesis and renal function. Ang II downstream signaling JNK pathway is one of the major signaling cassettes of the MAPK signaling pathway. In our experiment, I tested this antibody for the time-course effect of JNK protein levels in HASMC cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lysate samples.

Primary Incubation

1:1000 dilution in 4% skim milk prepared in 1X TBST

Blocking Agent

1% BSA prepared in 1X TBST

Secondary Incubation

1:4000 4% skim milk prepared in 1X TBST

Tertiary Incubation

Nil

Detection

ECL method

Results Summary

Time dependent increased P-JNK levels compared to control group in non-canonical signaling pathway

DOI or PMID #

NA

Additional Notes

NA

Image Gallery

Summary

The Good

Great

The Bad

None

The Bottom Line

This antibody has a good signal and highly recommended to others.

Share your experience with other scientists. Write a Review! »

Join the discussion