Solid Mini Prep Kit that Requires some Tuning

University of Toronto
Chemical/Biomedical Engineering
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

Mini prep kit

Catalog Number:

27104

We broadly study the mechanisms responsible for establishing and maintaining immune tolerance in the periphery

Experimental Design and Results Summary

Application

Preping DNA from transformed bacterial cells for cloning experiments

Starting Material

Transformed, chemically competent bacteria

Tips

Preheating the elution buffer can sometimes improve yields

Results Summary

Typically provides reasonable (150-200 ng/ul) yields with high purity but can occasionally be inconsistent with abnormally low yields when following the protocol as written without further optimization

DOI or PMID #

N/A

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Summary

The Good

Affordable

The Bad

Protocol can be inconsistent as written and may require some optimization

The Bottom Line

A decent quality mini prep kit when optimizing yield to the maximum possible is not required

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