Great for Intracellular Flow Cytometry

University of Toronto
Chemical/Biomedical Engineering
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBiosciences

Product Name:

Foxp3 / Transcription Factor Staining Buffer Set

Catalog Number:

00-5523-00

We broadly study the mechanisms responsible for establishing and maintaining immune tolerance with a specific focus on the transcription factors and signaling cascades involved.

Experimental Design and Results Summary

Application

Fixing and permeabilizing cells for intracellular staining of Foxp3 and Aire

Starting Material

Thymus, Lymph nodes, Spleen

Tips

Sometimes fixing in PFA before proceeding to using this fix/perm kit helps to reduce background staining.

Results Summary

Typically great staining above background for intracellular transcription factors with convenient and easy to use reagents.

DOI or PMID #

N/A

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Summary

The Good

Very useful for staining intracellular transcription factors and proteins that are hard to stain using traditional methods.

The Bad

More expensive

The Bottom Line

Great for intracellular staining for proteins that are traditionally hard to visualize via flow.

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