Great Way to Look at Affinity for IL-7 Differentiation

Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

CD127 Monoclonal Antibody APC-eFluor 780

Catalog Number:

47-1271-82

Clone Number:

A7R34

Our lab is interested in looking at response of T cells after any type of acute infection. For flow, we would like to look at expression of CD127 to distinguish between differentiated cells or non-differentiated.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine T Cells

Primary Incubation

1:500 in FACS Buffer

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow BD Celesta

Results Summary

Since we are interested in looking at effector and memory T cells, our interest strongly lie on CD127 and KLRG1. By looking at CD127-KLRG1+ populations we can distinguish "memory-like" and "effector-like" cells upon infection.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Once titrated, the antibody works really well each time.

The Bad

The APC can easily bleed into other channels

The Bottom Line

This is definitely a must if your lab is interested in looking at IL7 and T cell differentiation.

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