Easy and Reliable Protein Quantification Assay

Emory University
Pediatrics
Graduate Student

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Quality of Results

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Company:

ThermoFisher Scientific

Product Name:

Pierce BCA Protein Assay Kit

Catalog Number:

23225

Easy and reliable protein quantification assay

Experimental Design and Results Summary

Application

Protein Quantification

Starting Material

Plasma, BAL, culture media

Tips

Works well for a variety of sample types. Dilution may be needed.

Results Summary

I used this assay to measure total protein concentration in plasma and BAL samples. It is run in 96 well plate format in duplicate, and is reliable with small sample volumes. Reliable standard curves are obtained using BSA.The assay can be completed in 1 hour and is read in a plate reader at 562 nm.

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Summary

The Good

Fast and reliable assay requiring minimal sample

The Bad

may require sample dilutions to ensure they fall within the range of the standard curve

The Bottom Line

Great assay for measuring protein

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