Excellent Antibody for Tubulin b3/Tuj1 from BioLegend

ICGS
University of Birmingham
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-tubulin b 3

Catalog Number:

801213

Clone Number:

Monoclonal

Our research is to investigate the role of autophagy in (hPSCs) human pluripotent stem cell-derived cellular platforms, relative to neurodegenerative and infectious diseases. We are focusing on biomedical implications of autophagy in cell type-specific context. We utilized this antibody for IF staining of iPSC-derived neurons and were very satisfied with the results.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human induced pluripotent stem cell-derived neurons

Primary Incubation

1:200 overnight at 4 degrees

Blocking Agent

3% BSA in PBS

Secondary Incubation

1:1000 anti-mouse at RT for 60 mins

Tertiary Incubation

Not required

Detection

Immunofluorescence

Results Summary

The antibody produced very accurate staining and was easy to work with.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining in human stem cell-derived neurons

The Bad

No downsides

The Bottom Line

Would recommend for characterizing neuronal cultures. Can be combined with other assays and fluorescent labels.

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