MAP2 Antibody of Good Quality (Sigma)

ICGS
University of Birmingham
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

MilliporeSigma

Product Name:

Anti-MAP2 antibody

Catalog Number:

M3696

Clone Number:

Polyclonal

Browse Similar Products: MAP2 Immunofluorescence Antibodies

Our research is to investigate the role of autophagy in (hPSCs) human pluripotent stem cell-derived cellular platforms, relative to neurodegenerative and infectious diseases. We are focusing on biomedical implications of autophagy in cell type-specific context. We used this antibody for IF to characterize iPSC-derived neurons and it worked beautifully.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human induced pluripotent stem cells

Primary Incubation

Standard dilution we use is 1:1000 overnight at 4 degrees

Blocking Agent

5% goat serum

Secondary Incubation

Rabbit 1:4000, RT, 60 mins

Tertiary Incubation

Not required

Detection

Immunofluorescence

Results Summary

Would recommend this antibody as the staining worked well, with no background staining, and no other cells labeled other than neurons.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Worked well with no optimization needed.

The Bad

No cons.

The Bottom Line

Would definitely recommend

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