Great Antibody for Actin

February 13, 2019

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

β-Actin (13E5) Rabbit mAb

Catalog Number:

4970

We commonly use beta-actin as a loading control for immunoblot across all treatment conditions. The antibody is easy to use and works well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lymphocytes

Primary Incubation

1:10000 in 4% BSA in TBST

Blocking Agent

5% dry milk in TBST

Secondary Incubation

Anti-rabbit HRP in 5% milk in TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

As long as the loading is similar, beta-actin always shows same intensity

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very easy to use

The Bad

None

The Bottom Line

Great antibody for loading control. Can be reused multiple times.

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