Cell Signaling Technology
β-Actin (D6A8) Rabbit mAb
8457
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Browse Similar Products: Beta-Actin Western Blot Antibodies
We routinely perform western blots for all types of cells (macrophages, tumor, T cells). To normalize total protein content in certain experiments, we use actin as a control in our blots. This is a nice antibody with great specificity.
Western Blot
Murine macrophages
1:10000 in 4% BSA/TBST
5% dry milk/TBST
1:5000 Rabbit IgG HRP in 5% dry milk/TBST
None
Invitrogen ECL Pico
We are able to detect total beta-Actin levels in our lysates. The signal is quite strong since we detect it within seconds of exposure.
28877476
Be aware, the antibody is not best to reuse after 5 times or so since signal fades fast.
Strong signal within seconds if antibody is more fresh but not too bad since 1:1000 dilution
Can't reuse antibody mix many times
Nice antibody to detect beta-Actin for a loading control. Great specificity also!